The Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) were derived from primary mouse aortic endothelial cells isolated from the thoracic and abdominal aortas of control mice. The primary cells were immortalized using polyoma middle-sized T-antigen (PmT) method. The Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) is able to retain the properties and phenotype of the parental cells including the expression of common markers of endothelial cells including PECAM1, eNOS, VE-cadherin, and von Willebrand Factor. When tested for the functional responses to physiologically relevant shear stresses, such as laminar shear, the Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) still maintained the ability to form tubes as expected from primary endothelial cells. With these characteristics, the Immortalized Mouse WildType Aortic Endothelial Cells (iMAEC-WT) is a useful model in cardiology, particularly in studying vascular biology and pathobiology in vitro. These cells provide solution in difficulty in maintaining mouse aortic endothelial cells in multiple passages without phenotypic drift.
Cell Type
Immortalized Cells
Disclaimer
1. For for-profit organizations and corporations, please contact orders@biohippo.com for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at licensing@abmgood.com. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Expression Level
PECAM1, eNOS, VE-cadherin, von Willebrand Factor
Quantity
1x106 cells / 1.0 ml
Tissue
Heart
Morphology
Elongated
Population Doubling
20 - 30 hours
Propagation
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, Non-Essential Amino Acids (100X) (TM068) to a final concentration of 1X, and ECGS (Corning) to a final concentration of 50 µg/ml.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: 37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.Recommended split ratio of 1:2. Perform a PBS wash twice before using warmed 0.25% Trypsin-EDTA (TM050) to subculture. Cells must be seeded over 20,000 cells/cm2. Cells seeded at lower densities will result in a significant reduction in cell growth.
Quality Control
1) FACS cell sorting; 2) Characterization by Dil-Ac-LDL staining and immunostaining
Reviews of Immortalized Mouse Urogenital Sinus Mesenchymal Cells (UGSM-2)