Immortalized Mouse Promyelocyte Cells (MPRO C.2) - SV40

Mfr.No.	T0686
Description	Immortalized Mouse Promyelocyte Cells (MPRO C.2) - SV40 were generated through the infection of freshly harvested mouse bone marrow cells with the LRAR?403SN retroviral vector. Immortalized Mouse Promyelocyte Cells (MPRO C.2) - SV40, when treated with retinoic acid, terminally differentiate into mature neutrophils. Immortalized Mouse Promyelocyte Cells (MPRO C.2) - SV40 are GM-CSF dependent. Research uses of Immortalized Mouse Promyelocyte Cells (MPRO C.2) - SV40 include studying the effect of retinoic acid in the differentiation of neutrophils. Characterization via growth pattern analysis, Northern analysis, and transient expression analysis.
Cell Type	Immortalized Cells
Biosafety Level	2
Depositor	CELLL, L.L.C
Disclaimer	1. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 ?g, Cat.# C207, $450.00) or cell lysate (100 ?g, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 5. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Donor History	Male, 6 weeks
Expression Region	Express high levels of retroviral mRNA harboring the truncated RAR?403 sequence, mouse neutrophil-specific antigen 7, positive staining for chloroacetate esterase
Expression Level	Androgen receptor, Nkx3.1, beta-catenin, CK8, CK18 and vimentin
Growth Properties	Suspension, round; may form aggregates
Growth Conditions	PriCoat?T25 Flasks (G299) are recommended for optimal cell culture.?riGrow V (TM015)? 20% Horse Serum +?25 ng/ml?ouse IL-3 (Z200145)? 2.5 ng/ml Recombinant Mouse GM-CSF (CSF2) (Z200075) + 10ng/ml Recombinant Human IL1B (Z100395) + 20ng/ml Recombinant Human IL6 (Z100555) +?% Penicillin/Streptomycin Solution (G255), 37.0?, 5% CO?.Note: allow cells to recover during the first 24-48 hours post-thaw.
Quantity	1x106 cells / 1.0 ml
Tissue	Bone Marrow
Morphology	Cuboidal
Notes	1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130?, preferably in liquid nitrogen vapor phase storage, until ready for use.To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130? or in liquid nitrogen vapor phase. Do not store at -70?, as it will result in loss of viability.
Organism	Mouse (M. musculus)
Population Doubling	23 - 33 hours
Species	Mouse (M. musculus)
Propagation	Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow III medium available from abm (TM003). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0
Cryopreservation	Cryopreservation Medium (TM024), or complete growth media with 10% DMSO.
Quality Control	1. Western blot was used to confirm the presence of E1A and p53DD gene in the immortalized cell line. 2. Western blot was used to check tissue-specific markers.

Applications Range	Research Use Only.

Shipping	Ship with dry ice.
Product Format	Frozen
Product Use	This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Storage	Vapor phase of liquid nitrogen, or below -130?.

Thawing Protocol	1. Thaw cells quickly in a 37? water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. 2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes. 4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask. 5. Incubate the cells at the recommended conditions.
Subculture Protocol	1. Simply add fresh complete media directly to the culture. Do not allow cell density to exceed 1x10? cells/ml. 2. Alternatively, replace complete growth media by centrifugation and re-suspend the cell pellet in fresh complete media, and add appropriate aliquots of the cell suspension to new culture vessels, as desired. 3. Incubate the cells at the recommended conditions.

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHC10900815	T0686	1x10^6 cells / 1.0 ml	-		Add to Quote

Immortalized Mouse Promyelocyte Cells (MPRO C.2) - SV40

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Immortalized Mouse Promyelocyte Cells (MPRO C.2) - SV40

Rate it now..!!