cAMP assay for Gi-coupled human glutamate receptor, metabotropic 4 (GRM4).
HEK293-CNG-Slcla3 cells (AG-10200-238) without transfected GRM4 are used as a negative control.
Gene Name
Human Glutamate Receptor, Metabotropic 4 (GRM4)
Cell Origin
Human
Culture Medium
Growth medium: 90% DMEM with Glutamax, 10% FBS, 250 µg/ml G418, 1 µg/ml puromycin and 5 µg/ml blasticidin
Freezing medium: 10% DMSO, 90% complete medium
No Description Available.
Figure 1. Response of ACTOne GRM4 cell line & parental cell line to L-(+)-2-Amino-4-phosphonobutyric acid. ACTOne GRM4 cells and parental cells (AG-10200-238) were plated overnight in 20 ml culture medium on a BD Biocoat 384 well plate. The next day, cells were dye-loaded with 20 ml/well of 1X Dye-loading solution (ACTOne Membrane Potential Assay Kit). After 2 hours of incubation at room temperature, two readings were obtained prior to and 45 min after the addition of L-(+)-2-Amino-4-phosphonobutyric acid. Ratios of the two readings (F/F0) are plotted in the figure. A. Dose response curve of L-(+)-2-Amino-4-phosphonobutyric acid in ACTOne GRM4 cell line. EC50 = 0.80 mM in the presence of PDE inhibitor Ro20-1724 and b-adrenoceptor agonist isoproterenol. B. Parental cells do not respond to L-(+)-2-Amino-4-phosphonobutyric acid.
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