Recombinant HL-60 cells constitutively expressing the firefly (Photinus pyralis) luciferase gene under the control of a CMV promoter.
Mycoplasma testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Host Species: human
Supplied as Each vial contains 2 x 106 cells in 1 ml of cell freezing medium (Biohippo, #79796)
Background
HL- 60 cells are an immortal human cell line derived from blood lymphocytes from a patient presenting acute promyelocytic leukemia (an aggressive leukemia). HL-60 cells can be differentiated through the myeloid pathway to become neutrophils by using DMSO, DMF, and other inducers. This cell line can be used in immune disorder and immunology research. The signal generated by the firefly luciferase is proportional to cell numbers.
Application
Use as an internal control in CAR-T co-culture killing assaysIn vitro and in vivo bioluminescence imaging
Host Cell
HL-60
Shipping Temperature
-80°C (dry ice)
Note
License Disclosure: Visit Biohippobioscience.com/license for the label license and other key information about this product. Troubleshooting Guide: Visit Biohippobioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email orders@biohippo.com. Warning: Avoid freeze/thaw cycles.
Storage Stability
Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.