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CRISPRa (SAM) Jurkat Cell Pool

SKU : BHC18200077

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BPS Bioscience

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Mfr.No.	78080
Description	This cell line has been engineered for use with the CRISPR Synergistic Activation Mediator (SAM) system to induce transcriptional activation and expression of any gene of interest. Cells stably express a mutated dCas9 (Streptococcus pyogenes CRISPR associated protein 9), lacking any endonuclease activity, fused to VP64, a transcriptional activator. Stable dCas9-VP64 expression is maintained with Blasticidin resistance. Cells also stably express P65 (Transcription Factor p65, or Nuclear Factor NF-kappaB p65) and HSF1 (Heat Shock Factor 1) fused with an MS2 tag, which is maintained with Hygromycin resistance. When these cells are transfected with an MS2-tagged sgRNA targeting the promoter region of the gene of interest, dCas9-VP64 and MS2-P65-HSF1 are recruited to the site in the genomic DNA and begin transcription, inducing expression of the desired gene. Host Species: human Supplied as Each vial contains ~ 2 x 10^6 cells in 1 ml of 10% DMSO in FBS.
Application	1. Quickly generating CRISPR-activated cell pools or cell lines in Jurkat cells. 2. Implementing sgRNA CRISPRa screens in Jurkat cells.
Host Cell	Jurkat
Species	Human
Synonyms	Synergistic Activation Mediator cells, dCas9 cell pool
Shipping Temperature	-80°C (dry ice)
Note	The CRISPR/CAS9 technology is covered under numerous patents, including U.S. Patent Nos. 8,697,359 and 8,771,945, as well as corresponding foreign patents applications, and patent rights.License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of Biohippo products to your laboratory. Biohippo does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact orders@biohippo.com for details. Publications using this cell line should reference Biohippo Inc. Warning: Avoid freeze/thaw cycles.

Storage Stability	Store in liquid nitrogen immediately upon receipt.

Reference	Konermann, S., et al. (2015). Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 517(7536): 583-588.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200077	78080	2 million cells	$874.50		Add to Cart

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