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Cas9-Expressing HeLa Cell Pool

SKU : BHC18200089

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BPS Bioscience

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Mfr.No.	78161
Description	Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing HeLa cells can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines. Mycoplasma testing: This cell line has been screened using the MycoAlert Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control. Host Species: human Supplied as 2 vials of frozen cell pools. Each vial contains 2 x 10^6 cells in 1 ml of 10% DMSO
Application	1. Quickly generating knock-out cell pools or cell lines in HeLa cells. 2. Implementing sgRNA screens in Cas9-expressing HeLa cells.
Host Cell	HeLa
Species	S. pyogenes
Synonyms	SpCas9 cell pool, CRISPR cell pool, Cas9 cell pool, HeLa cell pool
Shipping Temperature	-80°C (dry ice)
Note	License Disclosure: Visit Biohippobioscience.com/license for the label license and other key information about this product.Troubleshooting Guide: For all questions, please email orders@biohippo.com.The CRISPR/CAS9 technology is covered under numerous patents, including U.S. Patent Nos. 8,697,359 and 8,771,945, as well as corresponding foreign patents applications, and patent rights. Warning: Avoid freeze/thaw cycles.

Storage Stability	Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHC18200089	78161	2 vials	$874.50		Add to Cart

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