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Styrene - Maleic Acid Copolymer 3:1 Free Acid - 500 mg

SKU : BHE16300446

This product includes 500 mg of SMA Copolymer 3:1 free acid

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Mfr.No.	SMA31-500MG
Description	Styrene - maleic acid (SMA) copolymers are broadly used for function and structure studies of membrane proteins. The significant advantages of using SMA copolymers include (1) generating a detergent-free system and (2) forming bilayer nanodiscs with phospholipids. Application of SMA copolymers opens an avenue of membrane protein extraction from cell membranes and proteoliposomes in the absence of detergent. The extracted membrane proteins are stabilized in the nanodiscs that mimic the bilayer structure of lipids in nature. The membrane proteins in nanodiscs can be purified and employed in biochemical, biophysical and biological experiments. For example, the nanodiscs can be used for structure studies of membrane proteins by electronic microscopy (EM). Typically SMA copolymers 2:1 forms nanodiscs in a size about 30 nm while SMA copolymers 3:1 forms nanodiscs in a size about 10 nm. The ideal pH for nanodisc formation is between pH 7.0 – 8.0. Styrene - Maleic Acid Copolymer 3:1 Free Acid is the free acid form of SMA Copolymer with a molar ratio of styrene to maleic acid of 3:1. The copolymer molecular weight is 7.8 kDa. The free acid form of SMA copolymer is soluble in a pH 8.5 buffer. The package size is 100 mg, 500 mg, 5000 mg and 25 g. Styrene - Maleic Acid Copolymer 3:1 Sodium Salt is the sodium salt form of SMA copolymer with a molar ratio of styrene to maleic acid of 3:1. The copolymer molecular weight is 8.6 kDa. The salt form of SMA copolymer is soluble in pure water. The package size is 100 mg, 500 mg, 5000 mg and 25 g. Styrene - Maleic Acid Copolymer 3:1 Solution, pH 7.6 – 10 ml includes 10 ml of 5% Styrene - Maleic Acid Copolymer 3:1 in 0.1 M Tris-HCl, pH 7.6. Preparation of 5% SMA copolymer stock solution from the solid polymer A convenient way to make nanodiscs is to use a stock solution of 5 % SMA copolymer. The pH is adjusted to 7.6 which is optimal for nanodisc formation. The solution is filtrated through a 0.8 um filter and stored in a 4ºC refrigerator or -20 ºC freezer. Note: The buffer used for SMA copolymer nanodiscs should be between pH 6 – 9, preferably pH 7 – 8. A lower pH will result in precipitation of the copolymer and a higher pH will destabilize the nanodisc structure. Formation of nanodiscs from cell membranes Incubate 15 mg of cell membrane in 5 ml of 50 mM Tris-HCl, pH 7.6, 0.5 M NaCl, and 10 % glycerol with 5 ml of 5 % SMA copolymer, pH 7.6 by gentle agitation at room temperature overnight. Formation of nanodiscs from Proteoliposomes Incubate proteoliposomes composed of 15 mg of lipids and reconstituted membrane proteins in 5 ml of 50 mM Tris-HCl, pH 7.6, 0.5 M NaCl, and 10 % glycerol with 5 ml of 5 % SMA copolymer, pH 7.6 by gentle agitation at room temperature overnight.
Nanodiscs	SMA copolymer 3 to 1

Shipping Temperature	Room temperature
Purification	Purification of nanodiscs Nanodiscs can be dialyzed using dialysis membranes to remove small molecules and concentrated by ultrafiltration. Size exclusion chromatography (SEC) can be employed for purification of nanodiscs. Protein purification SEC or affinity column chromatography can be employed for membrane protein purification of the discoidal membrane proteins. However, complete solubilization with a proper detergent to the size of molecular level is often required to gain high purity proteins. The chromatographically purified proteins in detergent micelles are reconstituted in liposomes or nanodiscs.

Product Manual	Datasheet

Reference	Jonas M. Dörr et al, The styrene–maleic acid copolymer: a versatile tool in membrane research. Eur. Biophys. J. (2016) 45:3 - 21.

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Combination SKU	Supplier No.	Specifications	Size	Your price	Quantity
BHE16300446-1	SMA31-500MG	Free Acid	500 mg	$478.98		Add to Cart
BHE16300446-2	SMA31-5000MG	Free Acid	5000 mg	$2280.85		Add to Cart
BHE16300446-3	SMA31-100MG	Free Acid	100 mg	$191.59		Add to Cart
BHE16300446-4	SMA31-25G	Free Acid	25 g	$4689.21		Add to Cart
BHE16300446-5	SMA31S-G	Sodium Salt	25 g	$4689.21		Add to Cart
BHE16300446-6	SMA31S-500MG	Sodium Salt	500 mg	$478.98		Add to Cart
BHE16300446-7	SMA31S-5000MG	Sodium Salt	5000 mg	$2280.85		Add to Cart
BHE16300446-8	SMA31S-100MG	Sodium Salt	100 mg	$191.59		Add to Cart
BHE16300446-9	SMA31-10ML	Solution	10 ml	$495.61		Add to Cart

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