Triglycerides (TG) can be hydrolyzed by lipoprotein lipase into glycerol and free fatty acids. Glycerol produces glycerol-3-phosphate and ADP under the catalysis of glycerol kinase (GK). Glycerol-3-phosphate produces hydrogen peroXide under the action of glycerol phosphate oXidase (GPO). In the presence of 4-aminoantipyrine and phenol, hydrogen peroXide is catalyzed by peroXidase (POD) to produce quinones which is proportional to the content of TG.
Research Area
Lipids metabolism
Dilutions
Itis recommended to take 2~3 samples with expected large difference to dopre-experiment before formal experiment and dilute the sample according to theresult of the pre-experiment and the scavengingcapacity detection range (10-50%). The recommended dilution factor for different samples is as follows(for reference only): Sample type Dilution factor, Human serum Dog serum Rat serum Cynomolgus monkey serum 10% Rat spleen tissue homogenate 10% Mouse liver tissue homogenate 10% Rat kidney tissue homogenate 10% Rat lung tissue homogenate 1. Note: Thediluent is double distilled water.