The widespread distribution in vivo and versatility of glutathione has attracted more and more attention of researchers. The glutathione plays a direct or indirect role in many significant organism phenomena and therefore involves various research topics such as enzymatic reaction mechanisms, biosynthesis of macromolecules such as proteins and DNA, intermediate metabolism, radiation, tumor, immunization, environmental toxins and aging, etc. In particular, many research topics have been involved in the defense of reactive oxygen species and free radicals in recent years. For the determination of biological samples, especially the concentration change of GSH and GSSG in serum (plasma) is an important indicator of the body oxide.
Description
The total glutathione and oxidized glutathione content in tissues and body fluids are determined by the cycling reaction of DTNB.
Description
Biochemical
Assay Type
Biochemical kit
Detection Instrument
Microplate reader
Detection Method
Colorimetric method
Detection Range
0-100μmol/L
Experiment Instruments
Microplate reader
Inter-cv (%)
5.32
Intra-cv (%)
2.8
Manual
Recovery
96
Sensitivity
0.1μmol/L
Target
T-GSH
Storage
Reagent 1(A): 4℃
Reagent 1(B): 4℃
Reagent 2(A): -20℃
Reagent 2(B): -20℃
Reagent 3(A): -20℃
Reagent 3(B): 4℃
Reagent 4: 4℃
Reagent 5(A): 4℃
Reagent 5(B): 4℃
Reagent 6: 4℃
Reagent 7: 4℃
Reagent 8: 4℃
Valid Period
3 months, 3
Manual
Reviews of Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit