JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
orders@biohippo.com

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Assay Kits

Sucrose Fluorometric Assay Kit

SKU : BHE10308178

Contact us to order

Tel

+1 866.986.9598

Email

orders@biohippo.com

Get a Quote

Request More Infos

Elabscience Biotechnology Co.,Ltd

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

orders@biohippo.com

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	E-BC-F042
Assay Time	70 min
Assay Type	QuantitatIVe
Inter-cv (%)	6.5
Intra-cv (%)	2.3
Manual	Data Sheet
Recovery	96
Sensitivity	0.15 umol/L
Test Principle	Sucrose can be hydrolyzed by sucrase to produce glucose under acidic conditions, which is catalyzed by glucose oXidase to produce hydrogen peroXide. In the presence of HRP (horse radish peroXidase), hydrogen peroXide reacts with the fluorescent probe to form red fluorescent substance. The sucrose content can be calculated by measuring the fluorescence intensity at the eXcitation wavelength of 535 nm and the emission wavelength of 590 nm.

Research Area	Others

Dilutions	The optimalsampling volume are different for differentspecies, the SOD also are different for different samples. It is recommended totake 2~3 samples to do a pre-experiment, diluting a series of diluent anddetermine the dilution factor when the SOD inhibition ratio is 15%~55% ( the optimal inhibition ratio is the range of 25%~45% ) before formal experiment.The recommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor, Mouse serum 5-10, Rat serum 6-15, Urine 2-3, Human hydrothorax 2-3, 10% Mouse liver tissue homogenate 100-200, 10% Mouse brain tissue homogenate 20-30, 10% Mouse kidney tissue homogenate 50-120, 10% Rat kidney tissue homogenate 50-120, HepG2 cells (5.21mgprot/mL) 15-25, Cell culture supernatant 1. Note: The diluent is normalsaline (0.9% NaCl).
Storage	-20°C
Valid Period	6 Months

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHE10308178	E-BC-F042	96T	$350		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.