Fast and sensitive. Use of 10 uL sample. Linear detection range 0.5 to 6 U/L for 30 min reaction at 25°C. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at 0 and 30 minutes. Room temperature assay. No 37°C heater is needed.High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Intended Use
FL530/585nm
Detection Principle
0.5 U/L
Shelf Life
6 months
Specifity
For quantitative determination of diamine oxidase in biological samples (e.g. serum, plasma etc)