High sensitivity and wide linear range. Use 10 uL sample. Detection limit of 0.02 U/L (20 min reaction).Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ALP activity within 20 minutes.Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.
Intended Use
FL360/450nm
Detection Principle
0.02 U/L
Shelf Life
2 years
Specifity
For quantitative determination of alkaline phosphatase (ALP) activity using stable 4-methylumbelliferyl phosphate substrate.
Reference
Domazetovic, V., et al (2020). Blueberry juice antioxidants protect osteogenic activity against oxidative stress and improve long-term activation of the mineralization process in human osteoblast-like saos-2 cells: Involvement of sirt1. Antioxidants (Basel, Switzerland), 9(2). Assay: Alkaline Phosphatase in human bone cells.
Laura, BP et al. (2015). Lasting effects of butyrate and low FM/FO diets on growth performance, blood haematology/biochemistry and molecular growth-related markers in gilthead sea bream (Sparus aurata). Aquaculture. 454 (2016) 8-18. Assay: Alkaline phosphatase in fish plasma.
Maryam, RR et al. (2015). The role of dentin matrix protein 1 (DMP1) in regulation of osteogenic differentiation of rat dental follicle stem cells (DFSCs). Arch Oral Biol. 60(4): 546-556. Assay: Alkaline phosphatase in rat cells.
Ballester-Lozano GF et al. (2015). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. Br J Nutr. 114(5):713-26. Assay: alkaline phosphatase enzyme activity in fish plasma.
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