Fast and sensitive. Linear detection range (20 uL sample): 1 mg/mL (0.17 mM) to 100 mg/mL (17 mM) urea in 96-well plate assay.Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 5 minutes. Room temperature assay. No 37°C heater is needed.High-throughput. Homogeneous "mix-incubate-measure" type assay.Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Intended Use
OD557nm
Detection Principle
1 mg/mL (0.17 mM)
Shelf Life
6 months
Specifity
For quantitative enzymatic determination of urea.
Reference
Tokunaga, A.,et al (2020). Effect of chronic kidney disease on hepatic clearance of drugs in rats. Biological & Pharmaceutical Bulletin, 43(9), 1324-1330. Assay: Urea in rat serum.
Yang, W.,et al (2017). Epigallocatechin-3-gallate decreases the transport and metabolism of simvastatin in rats. Xenobiotica; the Fate of Foreign Compounds in Biological Systems, 47(1), 86-92. Assay: Urea in rat plasma.
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