Sensitive and accurate. Detection range 0.6 - 200 uM in 96-well plate.Rapid and reliable. Using an optimized VCl3 reagent, the time required for reduction of nitrate (NO3-) to nitrite (NO2-) is 10 min at 60°C. Simple and high-throughput. The procedure involves mixing sample with three reagents, incubation for 10 min at 60°C and reading the optical density. Can be readily automated to measure thousands of samples per day.
Intended Use
OD540nm
Detection Principle
0.6 uM
Shelf Life
6 months
Specifity
For quantitative determination of nitric oxide (nitrate/nitrite) and evaluation of drug effects on its metabolism.
Reference
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De la Cruz-Enriquez, J., et al. (2020). SARS-CoV-2 induces mitochondrial dysfunction and cell death by oxidative stress in leukocytes of COVID-19 patients. Authorea. Assay: Nitric Oxide in human plasma.
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Bonilla, E., Medina-Leendertz, S., Mora, M., Vielma, J. R., Atencio-Bracho, L., Bravo, Y. & Arcaya, J. L. (2017). Minocycline Protection against Paraquat Toxicity in Drosophila melanogaster. Toxicology International, 24(1), 65-73. Assay: Nitric oxidein plant tissues.
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Lipari et al (2011). Assay Of Nitric Oxide Metabolites In The Culture Media Of human Preimplantation Embryosus. US 2011/0009689 A1. Assay: Nitric Oxide in human tissue culture.
Zeng Q, et al (2011). Control of Cervicovaginal HPV-16 E7-Expressing Tumors by the Combination of Therapeutic HPV Vaccination and Vascular Disrupting Agents. Hum Gene Ther. 22(7):809-19. Assay: Nitric oxidein human tissue.
Alonso F, et al (2010). Loss of connexin40 is associated with decreased endothelium-dependent relaxations and eNOS levels in the mouse aorta. Am J Physiol Heart Circ Physiol. 299(5):H1365-73. Assay: Nitric oxidein mouse aorta.
Jawed H, et al (2010). N-(2-hydroxy phenyl) acetamide inhibits inflammation-related cytokines and ROS in adjuvant-induced arthritic (AIA) rats. Int Immunopharmacol. 10(8):900-5. Assay: Nitric oxidein rat serum and plasma.
Labib, HM, et al (2010). The Role of Oxidative Stress Markers and Nitric Oxide Levels in the Pathogenesis of Glaucoma. Austr. J. Basic and Applied Sci 4(8): 3553-3558. Assay: Nitric Oxide in human blood.
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Levy H, et al (2010). Characterization of brain lesions in a mouse model of progressive multiple sclerosis. Exp Neurol. 226(1):148-58. Assay: Nitric oxidein mouse tissue.
Smith MJ, et al (2010). Modulation of murine innate and acquired immune responses following in vitro exposure to electrospun blends of collagen and polydioxanone. J Biomed Mater Res A. 93(2):793-806. Assay: Nitric oxidein mouse tissue.
Carrero Y, et al (2009). Increased vascular endothelial growth factor expression, CD3-positive cell infiltration, and oxidative stress in premalignant lesions of the cervix. Cancer 115(16):3680-8. Assay: Nitric oxidein human cervix and serum.
Deshmukh SH, et al (2009). Ranolazine improves endothelial function in patients with stable coronary artery disease. Coron Artery Dis. 20(5):343-7. Assay: Nitric oxidein human tissue.
Lipari CW, et al (2009). Nitric oxide metabolite production in the human preimplantation embryo and successful blastocyst formation. Fertil Steril. 91(4 Suppl):1316-8. Assay: Nitric oxidein human tissue culture.
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Wang SH,et al (2008). Decreased inflammatory response in Toll-like receptor 2 knockout mice is associated with exacerbated Pneumocystis pneumonia. Microbes Infect. 10(4):334-41. Assay: Nitric oxidein mouse BALF.
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Hasegawa K, et al (2007). Role of asymmetric dimethylarginine in vascular injury in transgenic mice overexpressing dimethylarginie dimethylaminohydrolase 2. Circ Res. 101(2):e2-10. Assay: Nitric oxidein mouse tissue extracts.
Bolander FF. (2005). The compartmentalization of prolactin signaling in the mouse mammary gland. Mol Cell Endocrinol. 245(1-2):105-10. Assay: Nitric oxidein mouse mammary cells.
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