High sensitivity and wide linear range. Use 3 uL serum or plasma sample. The detection limit is 2 U/L, linear up to 200 U/L.Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of LDH activity within 30 minutes.Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.
Intended Use
OD565nm
Detection Principle
2 U/L
Shelf Life
6 months
Specifity
For quantitative determination of lactate dehydrogenase LDH activity and screen/evaluation of LDH modulators.
Ryan, E. L., Klopfenstein, J. J., & Kutzler, M. A. (2020). Intramammary antibiotics with complementary acupuncture decreases milk serum N-acetyl-beta-D-glucosaminidase concentrations in dairy cattle with subclinical mastitis. Reproduction in Domestic Animals, 55(12), 1747-1755. Assay: lactate dehydrogenase in bovine milk serum.
Haney, SL et al (2018). Preclinical investigation of a potent geranylgeranyl diphosphate synthase inhibitor. Investigational New Drugs. 36(5): 810-818. Assay: Lactate dehydrogenase in mice cells.
Berlingieri, S. (2017). Gain And Loss Of Progranulin Have Opposite Effects On Autophagy (Doctoral dissertation, The Open University). Assay: Lactate dehydrogenase in human liver cells.
Ghosh, Samit, et al. (2016). Nonhematopoietic Nrf2 dominantly impedes adult progression of sickle cell anemia in mice. JCI insight 1(4). Assay: Lactate dehydrogenase in mice plasma.
Higashiyama, M et al (2012).Indomethacin-induced small intestinal injury is ameliorated by cilostazol, a specific PDE-3 inhibitor. Scandanavian Journal of Gastroenterology 47(8-9):993-1002. Assay: Lactate dehydrogenase in mice intestinal cells.
Farina, F., et al. (2011). The acute toxic effects of particulate matter in mouse lung are related to size and season of collection. Toxicol Lett 202(3):209-17. Assay: Lactate dehydrogenase in mouse BALF.
Methods for treatment of oncological disorders using an epimetabolic shifter (coenzyme q10). US Patent Appl. 20110027247. Assay: Lactate Dehydrogenase in mouse cell.