High sensitivity and wide linear range. Use 20 uL sample. The detection limit is 2 U/L, linear up to 250 U/L.Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ?-glucosidase activity within 20 minutes.Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.
Intended Use
OD405nm
Detection Principle
2 U/L
Shelf Life
6 months
Specifity
For quantitative determination of ?-glucosidase activity and evaluation of drug effects on its metabolism.
Reference
Yang, W et al (2020). Increased ?-synuclein oligomerization is associated with decreased activity of glucocerebrosidase in the aging human striatum and hippocampus. Neuroscience Letters, 733, 135093. Assay: ?-glucosidase in human.
Yang, W et al (2020). ?-Syn oligomers incubated with Parkinson?s disease plasma promote neuron damage. International Journal of Clinical and Experimental Pathology, 13(8), 1995-2008. Assay: ?-glucosidase in human plasma.
Moon, JY et al (2015). Inhibition of cell growth and down-regulation of telomerase activity by amygdalin in human cancer cell lines. Animal Cells and Systems 19(5): 295-304. Assay: beta-Glucosidase in human cells.
Renchinkhand, G et al (2015). Identification of beta-Glucosidase Activity of Enterococcus Faecalis CRNB-A3 in Airag and Its Potential to Convert Ginsenoside Rb1 from Panax Ginseng. Journal of Food Biochemistry 40(1): 120-129. Assay: beta-Glucosidase in Enterococcus faecalis bacteria.
Liu, Z et al (2014). Mammalian expression levels of cellulase and xylanase genes optimised by human codon usage are not necessarily higher than those optimised by the extremely biased approach. Biotechnology Letters 36(11): 2169-2176. Assay: beta-Glucosidase in mammal cells.
Mallery SR, et al (2011). Effects of human oral mucosal tissue, saliva, and oral microflora on intraoral metabolism and bioactivation of black raspberry anthocyanins. Cancer Prev Res (Phila). 4(8):1209-21. Assay: beta-Glucosidase in human saliva.
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