Sensitive and accurate. Detection range 0.04 - 4% alcohol in 96-well plate assay.Convenient and high-throughput. The procedure involves adding a single working reagent, incubation for 8 min, adding a Stop Reagent, and reading the optical density. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.Versatility. Assays can be executed in 96-well plate or cuvet.
Intended Use
OD580nm (Chemical)
Detection Principle
0.0004
Shelf Life
12 months
Specifity
For quantitative determination of ethanol and alcohol metabolism.
Reference
Sawant, O. B., et al. (2020). Prenatal alcohol exposure and maternal glutamine supplementation alter the mTOR signaling pathway in ovine fetal cerebellum and skeletal muscle. Alcohol (Fayetteville, N.Y.), 89, 93-102. Assay: Ethanol in sheep blood.
Laksitorini, M. D., et al. (2020). Impact of Wnt/beta-catenin signaling on ethanol-induced changes in brain endothelial cell permeability. Journal of Neurochemistry. Assay: Ethanol in mouse tissue and human cells.
Nugent, B., Ali, S. S., Mullins, E., & Doohan, F. M. (2019). A Major Facilitator Superfamily Peptide Transporter From Fusarium oxysporum Influences Bioethanol Production From Lignocellulosic Material. Frontiers in microbiology 10:295. Assay: Ethanol in Fungus.
Contreras, M. L., de la Fuente-Ortega, E., Vargas-Roberts, S., Munoz, D. C., Goic, C. A., & Haeger, P. A. (2017). NADPH Oxidase Isoform 2 (NOX2) is involved in drug addiction vulnerability in progeny developmentally exposed to ethanol. Frontiers in Neuroscience, 11, 338. Assay: Ethanol in Sprague Dewley rats serum.
Duy, D. L., Suda, Y., & Irie, K. (2017). Cytoplasmic deadenylase Ccr4 is required for translational repression of LRG1 mRNA in the stationary phase. PloS one, 12(2), e0172476. Assay: Ethanol in E. coli media.
Kim HR, et al (2011). Raw starch fermentation to ethanol by an industrial distiller's yeast strain of Saccharomyces cerevisiae expressing glucoamylase and alpha-amylase genes. Biotechnol Lett. 33(8):1643-8. Assay: Ethanol in yeast starch.
Pisu MG, et al (2011). Effects of voluntary ethanol consumption on emotional state and stress responsiveness in socially isolated rats. Eur Neuropsychopharmacol.21(5):414-25. Assay: Ethanol in rat plasma.
Alam MZ,et al (2009). Production of bioethanol by direct bioconversion of oil-palm industrial effluent in a stirred-tank bioreactor. J Ind Microbiol Biotechnol. 36(6):801-8. Assay: Ethanol in plant oil-palm.
Lin, YY et al (2009). Protein acetylation microarray reveals that NuA4 controls key metabolic target regulating gluconeogenesis. Cell 136(6):1073-84. Assay: Ethanol in yeast culture medium.
Wang J, et al (2009). Altered pattern of Na,K-ATPase activity and mRNA during chronic alcohol consumption by juvenile and adolescent rats. Cell Mol Neurobiol. 29(1):69-80. Assay: Ethanol in rat blood.
Biggio F, et al (2007). Flumazenil selectively prevents the increase in alpha(4)-subunit gene expression and an associated change in GABA(A) receptor function induced by ethanol withdrawal. J Neurochem. 102(3):657-66. Assay: Ethanol in rat primary neuronal cultures, medium.
Khanna D, et al (2007). Inducible nitric oxide synthase attenuates adrenergic signaling in alcohol fed rats. J Cardiovasc Pharmacol. 50(6):692-6. Assay: Ethanol in rat serum.
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