In the presence of ADP, Pyruvate kinase can catalyze PEP to produce pyruvic acid, which will be transformed to lactic acid by LDH, meanwhile, NADH will be transformed to NAD.
Detection Range
1.3-250U/L
Experiment Instruments
Spectrophotometry (UV)
Inter-cv (%)
6.55
Intra-cv (%)
3.4
Manual
Recovery
101
Sensitivity
1.3 U/L
Target
PK
Storage
Reagent 1: 4℃
Reagent 2: -20℃
Reagent 3: 4℃
Reagent 4: -20℃
Reagent 5: -20℃
Valid Period
3 months, 3
Manual
Reviews of Pyruvate Kinase Colorimetric Assay Kit (Tissue and serum samples)