The content of protein carbonyl increased after oXidation, and the carbonyl group reacted with 2, 4-dinitrophenylhydrazine to form a reddish brown precipitate. The absorbance can be measured at 370 nm after the precipitation is dissolved. The carbonyl content can be calculated indirectly.
Research Area
OXidatIVe stress,Amino acids & proteins
Dilutions
It isrecommended to take 2~3 samples with expected large difference to dopre-experiment before formal experiment and dilute the sample according to theresult of the pre-experiment and the detection range (0.03-1.5mmol/L. Therecommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor, 10% Rat muscle tissue homogenate 2-4, 10% Rat liver tissue homogenate 2-4, 10% Mouse brain tissue homogenate 2-4, 10% Rat kidney tissue homogenate 2-4, 10% Rat lung tissue homogenate 2-4. Note: Thediluent is double distilled water.
Storage
2-8°C
Valid Period
6 Months
Reviews of Protein Carbonyl Colorimetric Assay Kit (Tissue And Serum Samples)