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Home / Assay Kits / ELISA Kits

Pig Myosin light polypeptide 6 (MYL6) ELISA Kit

SKU : BHE13706867

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DB Biotech, Inc.

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Mfr.No.	KTE80095
Alternative Names	MYL6 ; ESMLC ; LC17-GI ; LC17-NM ; LC17A ; LC17B ; MLC1SM ; MLC3NM ; MLC3SM ; myosin ; light polypeptide 6 ; alkali ; smooth muscle and non-muscle ; smooth muscle and non-muscle myosin alkali light chain
Background	Myosin light polypeptide 6 is a protein that in humans is encoded by the MYL6 gene. Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Genomic sequences representing several pseudogenes have been described and two transcript variants encoding different isoforms have been identified for this gene.
Categories	Elisa Kit
Description	This Pig Myosin light polypeptide 6 (MYL6) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL6.
Featured	Pig Myosin light polypeptide 6 (MYL6) ELISA Kit has high sensitivity and excellent specificity for detection of Pig MYL6. No significant cross-reactivity or interference between Pig MYL6 and analogues was observed.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Reactivity	Pig
Tag	MYL6
Description	This Pig Myosin light polypeptide 6 (MYL6) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL6 in samples. An antibody specific for MYL6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYL6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYL6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYL6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Accession Number	P60662
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich ELISA (quantitative)
Calibration Range	Please inquire
Detection Method	Colorimetric
Kit Components	• Pig Myosin light polypeptide 6 microplate • Pig Myosin light polypeptide 6 standard • Pig Myosin light polypeptide 6 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers
Limit Of Detection	Please inquire

Gene Id	106504064
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.

Shipping	Gel pack with blue ice.
Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Applications	ELISA

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SKU	Supplier No.	Size	Your price	Quantity
BHE13706867	KTE80095	48T	$380.54		Add to Cart
BHE13706867	KTE80095	96T	$580.54		Add to Cart
BHE13706867	KTE80095	96T*5	$2058.43		Add to Cart
BHE13706867	KTE80095	96T*50	$18258.43		Add to Cart

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