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Home / Assay Kits

Peptide Derivative Assay Kit

SKU : BHE16300257

This product includes 1 ml of 10 x C33 dye for 200 assays using 96-well plates. Cuvettes may also be used for the assay.

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Mfr.No.	PEPD200
Description	Many peptide derivatives or modified peptides have become peptide-based drugs for anticancer, anti-infection and other therapeutic applications. Peptide-based drugs often show higher specificity and lower side effects than regular small molecule-based drugs. The Peptide Derivative Assay Kit (Catalog number PEPD200) is designed for concentration measurement of various modified peptides in a DMSO solution. The assay is based on increase of fluorescence at 535 nm generated by binding of Dye C33 with peptides. The assay sensitivity is typically between 0.01 mg/ml and 0.1 mg/ml depending on the structure of the peptide derivatives. The assay is not compatible with amino acids, native peptides, thiol compounds such as DTT or metal chelators such EDTA. The Peptide Derivative Assay Kit (Catalog number PEPD200) includes 1 ml of 10 x C33 dye for 200 assays using 96-well plates. Cuvettes may also be used for the assay.

Product Manual	Datasheet
Protocols Description	ASSAY PROTOCOL The sample volume is 0.15 ml and the final volume is 0.2 ml in the 96-wellplate assay format. STANDARD CURVE 1. Dilute 50 µl of 10 x C33 dye 10 fold with ethanol to make 500 µl of 1 x C33 dye. 2. Prepare 150 µl of peptide solutions in DMSO in the wells of a black 96-well plate with a two[1]fold serial dilution from 0.5 mg/ml to zero in DMSO. 3. Mix 50 µl of 1 x C33 dye with 150 µl of the peptide solutions for 10 min. 4. Read the fluorescence at 535 nm (excitation at 485 nm). 5. Plot the fluorescence intensity Fc and the peptide concentration [Peptide] to generate the linear standard curve. Fc = a [Peptide] + b Where the Fc values are from experimental data, the a and b values are from the linear fitting between the Fc values and the peptide concentrations. UNKNOWN SAMPLES Follow the same procedure to measure the fluorescence intensity Fc values from the unknown samples. Calculate the peptide concentrations in the unknown samples using the Fc values from the unknown samples and the a and b values from the standard curve. [Peptide] = (Fc – b) / a

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SKU	Supplier No.	Size	Your price	Quantity
BHE16300257	PEPD200	200 assays	$302.17		Add to Cart

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