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Nucleic Acid Removal Kit

BHE16300400

This product includes 22 ml of NAR reagent 1 and 22 ml of Reagent 2. It is sufficient for treatment of 200 ml of cell lysate or protein solution.

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+1 866.986.9598
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orders@biohippo.com
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Reference Morrison K. D.et al, Unearthing the Antibacterial Mechanism of Medicinal Clay: A Geochemical Approach to Combating Antibiotic Resistance. Scientific reports, 6: 19043 (2016).
Product Manual Datasheet
Protocols Description PROTOCOLS
Remove Nucleic Acids during Cell Lysis
Cell lysis should be performed at 0 °C to 4 °C. Make sure all materials including the cell suspension, lysis buffer and NAR reagents are kept at 0 °C to 4 °C during the nucleic acid removal process.
(1) Mix one volume of NAR Reagent 1 with 9 volume of cell suspension.
(2) Break the cells.
(3) Remove the insoluble materials by centrifugation at 4 °C at 6000 x g for 20 min.
(4) Add one volume of NAR Reagent 2 and incubate the mixture on ice for 5 min. Remove the precipitate by centrifugation at 4 °C at 6000 x g for 20 min.
 
Note: For small scale tests using an Eppendorf benchtop centrifuge, centrifugation at the maximum speed (13000 rpm) for 2 minutes is sufficient.
 
Remove Nucleic Acids from a Protein Solution
Make sure all materials including the protein solution and NAR reagents arte kept at 0 °C to 4 °C during the nucleic acid removal process.
(1) Mix one volume of NAR Reagent 1 with 9 volume of the protein solution. Incubate the mixture on ice for 10 min.
(2) Remove the precipitate by centrifugation at 4 °C at 6000 x g for 20 min.
(3) Add one volume of NAR Reagent 2 and incubate the mixture on ice for 5 min. Remove the precipitate by centrifugation at 4 °C at 6000 x g for 20 min.
 
Protein Purification after Nucleic Acid Removal
The NAR Reagent 1 and Reagent 2 are ionic polymers. The final solution contains 0.45 M NaCl. The NAR regents can be easily removed in the process of protein purification. If ion exchange chromatography is used to remove the NAR reagents, the protein solution is dialyzed against 50 volumes of a low salt buffer for 4 hr to remove the salt and loaded on the column. The loaded column is washed with 5 - 10 column volumes of the low salt buffer and the protein is eluted with a salt gradient. If a gel filtration or affinity column is used, the dialysis step is not required.
SKU Supplier No. Size Your price Quantity
BHE16300400 NAR911 22 ml $144.01
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