Nitrotyrosine ELISA Kit

Mfr.No.	SKT-126
Alternative Names	Nitro tyrosine ELISA Kit, 3-Nitrotyrosine ELISA Kit, 3-Nitro-L-tyrosine ELISA Kit, 3 Nitrotyrosine ELISA Kit, 3-NT ELISA Kit, 3NT ELISA Kit, Nitro tyrosine ELISA Kit ; 3-Nitrotyrosine ELISA Kit ; 3-Nitro-L-tyrosine ELISA Kit ; 3 Nitrotyrosine ELISA Kit ; 3-NT ELISA Kit ; 3NT ELISA Kit
Categories	ELISA Kits
Description	Colorimetric detection of Nitrotyrosine
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Gross Weight (kg)	2
Net Weight (g)	500
Reactivity	Species independent
Scientific Background	Nitrotyrosine has been identified as a marker of inflammation and NO production. Nitrotyrosine is formed in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production. Since nitrotyrosine is a stable end product of peroxynitrite oxidation, assessment of its plasma concentration may be useful as a marker of NO-dependent damage in vivo. Since NOX is only an indicator for enhanced NO production, protein associated nitrotyrosine might be a more suitable marker for damage induced by reactive nitrogen intermediates derived from NO. Furthermore, most proteins have a longer half life in the circulation than NOX levels. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, celiac disease, rheumatoid arthritis, chronic renal failure and septic shock. In normal plasma low, undetectable, levels of nitrotyrosine are present. Nitrosylation of the amino acid tyrosine occurs both for free tyrosine and for protein bound tyrosine.
Size	1 Plate ; 1 vial/110µl ; 1 vial/75µl ; 1 vial/50mL ; 1 vial/13mL ; 1 vial/ 50mL ; 1 vial/13mL ; 1 vial/13mL ; 2
Adr Code	Non-hazardous
Assay Overview	1. Prepare standard and samples in the Sample and Standard Diluent. 2. Add 50 µL of prepared standards and samples in triplicate to appropriate wells. 3. Add 50 µL of the diluted antibody preparation to the appropriate wells. 4. Cover plate with Plate Cover and incubate at room temperature (20-25°C) for 1 hour. 5. Wash plate 4 times with 1X Wash Buffer. 6. Add 100 µL of TMB Substrate to each well. 7. Cover plate and develop the plate in the dark at room temperature for 30 minutes. 8. Add 100 µL of Stop Solution to each well. 9. Measure absorbance on a plate reader at 450 nm. 10. Plot the standard curve and calculate sample concentrations.
Assay Range	62.5 - 8000 nM
Assay Type	Competitive ELISA (Enzyme-linked Immunosorbent Assay)
Assay Type Abbreviation	Competitive
Component Number	SKC-126A ; SKC-126C ; SKC-126F ; SKC-0001 ; SKC-0002 ; SKC-0003 ; SKC-0004 ; SKC-0005 ; SKC-0009
Country Of Origin	Canada
Detection Method	Colorimetric Assay
Detection Range	62.5 - 8000 nM
Incubation Time	1 hour
Item	Nitrosylated BSA Coated Plate ; Nitrotyrosine Standard ; Nitrotyrosine HRP Conjugated Monoclonal Antibody ; Sample and Standard Diluent (Red) ; Nitrotyrosine Antibody Diluent (Blue) ; Wash Buffer Concentrate (10X) ; TMB Substrate ; Stop Solution ; Plate Cover
Method Type	Plasma, Serum, Cell Lysates, Urine
Number Of Samples	38 samples in duplicate
Platform	Microplate
Precision	Intra-Assay Precision: Three samples of known concentration were assayed thirty times on one plate. The intra-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be <10%. Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays. The inter-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be <15%.
Sensitivity	50 nM
Species Reactivity Abbreviations	ALL
Species Reactivity Medium	Species Independent
Trademark	MOLECULAR SIGNATURE®

Research Area	Cancer Oxidative Stress Cell Signaling Post-translational Modifications Nitration Neuroscience Neurodegeneration Alzheimer's Disease Parkinson's Disease
Tarriff Code	3822.00.1090
Unspsc Code	12352203

Shipping Temperature

Blue Ice

Description

Trademark: MOLECULAR SIGNATURE®
Platform: Microplate
Utility: ELISA kit used to quantitate Nitrotyrosine in samples.
kit-overview:

Component No.	Item	Quantity / Size
SKC-126A	Nitrosylated BSA Coated Plate	1 Plate
SKC-126C	Nitrotyrosine Standard	1 vial/110µl
SKC-126F	Nitrotyrosine HRP Conjugated Monoclonal Antibody	1 vial/75µl
SKC-0001	Sample and Standard Diluent (Red)	1 vial/50mL
SKC-0002	Nitrotyrosine Antibody Diluent (Blue)	1 vial/13mL
SKC-0003	Wash Buffer Concentrate (10X)	1 vial/ 50mL
SKC-0004	TMB Substrate	1 vial/13mL
SKC-0005	Stop Solution	1 vial/13mL
SKC-0009	Plate Cover	2

Incubation Time: 1 hour
Shipping Temperature: Blue Ice
Gross Weight (kg): 2
Net Weight (g): 500
Stock Product: Y
Country of Origin: Canada

Storage

4ºC

Storage Temperature

4ºC

Stock Product

Utility

ELISA kit used to quantitate Nitrotyrosine in samples.

Protocols Description	1. Prepare standard and samples in the Sample and Standard Diluent. 2. Add 50 µL of prepared standards and samples in triplicate to appropriate wells. 3. Add 50 µL of the diluted antibody preparation to the appropriate wells. 4. Cover plate with Plate Cover and incubate at room temperature (20-25°C) for 1 hour. 5. Wash plate 4 times with 1X Wash Buffer. 6. Add 100 µL of TMB Substrate to each well. 7. Cover plate and develop the plate in the dark at room temperature for 30 minutes. 8. Add 100 µL of Stop Solution to each well. 9. Measure absorbance on a plate reader at 450 nm. 10. Plot the standard curve and calculate sample concentrations.
Other Resources 1	Kit Booklet
Other Resources 2	MSDS
Other Resources 3	Calculations Worksheet