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Home / Assay Kits

NanoGram DNA Assay Reagent

BHE16300248

This product includes 5 ml of 10 x Reagent D. It is for 2000 assays using 384-well plates or 500 assays using 96-well plates. Cuvettes may also be used for the assay.

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Contact us to order
Tel
+1 866.986.9598
Email
orders@biohippo.com
Get a Quote
Ask Merchant
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Product Manual Datasheet
Protocols Description DNA STANDARD CURVE
1. Dilute the 10 x Reagent D with water 10 fold to make 1 x Reagent D.
2. Prepare a series of standard DNA solutions with concentrations from 0.5 to 100 µg / ml.
3. Mix 10 µl of the DNA solution with 100 µl of the 1 x Reagent D in the wells of a 96-well plate for 5 min.
 
Data Analysis
Plot the fluorescence intensity Fc and the DNA concentration [DNA] to generate the linear standard curve.
 
                                               Fc = a [DNA] + b
 
Where the Fc values are from experimental data, the a and b values are from the linear fitting between the Fc values and the DNA concentrations.
 
UNKNOWN SAMPLES
Follow the same procedure to measure the fluorescence intensity Fc values from the unknown samples. Calculate the DNA concentrations in the unknown samples using the Fc values from the unknown samples and the a and b values from the standard curve.
 
                                            [DNA] = (Fc – b) / a
SKU Supplier No. Size Your price Quantity
BHE16300248 NDA500 500 assays $234.76
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