Matrices listed below were spiked with certain level of EPO and the recovery rates were calculated by comparing the measured value to the expected amount of EPO in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-105
97
EDTA plasma(n=5)
86-101
93
heparin plasma(n=5)
89-102
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EPO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
EPO ELISA Kit allows for the in vitro quantitative determination of EPO concentrations in serum, plasma, tissue homogenates and other biological fluids.