Mouse CNP(C-type natriuretic peptide) ELISA Kit

Mfr.No.

E-EL-M0370

Antigen

CNP

Background

This kit uses competitive-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with Mouse CNP .During the reaction, Mouse CNP in the sample or standard competes with a fixed amount of Mouse CNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse CNP Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse CNP in the samples is then determined by comparing the OD of the samples to the standard curve.

Description

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	O.D	Average
1000	0.398 0.432	0.415
500	0.501 0.541	0.521
250	0.732 0.698	0.715
125	1.027 1.031	1.029
62.5	1.46 1.45	1.455
31.25	1.92 1.902	1.911
15.63	2.284 2.296	2.29
0	2.846 2.856	2.851

Specificity

This kit recognizes Mouse CNP in samples. No significant cross-reactivity or interference between Mouse CNP and analogues was observed.

Assay Time

2.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	4℃(shading light)
Stop Solution	1 vial, 10 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Colormetric

Detection Range

15.625--1000pg/mL

Linearity

Samples were spiked with high concentrations of Mouse CNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	99-114	91-104	98-115
1:2	Average (%)	106	98	105
1:4	Range (%)	85-98	93-105	106
1:4	Average (%)	92	99	106
1:8	Range (%)	85-99	91-105	96-111
1:8	Average (%)	90	97	102
1:16	Range (%)	85-100	91-106	93-105
1:16	Average (%)	91	99	100

Method Type

Competitive

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse CNP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse CNP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	51.20	150.10	345.40	54.10	162.30	320.10
Standard deviation	2.90	8.00	16.90	3.80	9.10	14.40
C V (%)	5.66	5.33	4.89	7.02	5.61	4.50

Recovery

The recovery of Mouse CNP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	87-99	94
EDTA plasma (n=5)	94-107	99
Cell culture media (n=5)	93-106	100

Sample Type

Serum, Plasma

Sensitivity

9.38 pg/mL

Manual

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SKU	Supplier No.	Size	Your price
BHE10302643	E-EL-M0370	96T	$609	Add to Cart
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BHE10302643	E-EL-M0370	96T*10	$5176.50	Add to Cart
BHE10302643	E-EL-M0370	48T	$487.20	Add to Cart

Mouse CNP(C-type natriuretic peptide) ELISA Kit

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