The body produce oxygen free radicals through the enzyme system and non-enzyme system, which can attack unsaturated fatty acid on biofilm, leading to lipid peroxidation and forming lipid peroxide, such as aldehyde group(MDA), keto group, hydroxyl group, carbonyl group etc. lipid peroxide can not only transform active oxygen to active chemical, but also enhance the effect of active oxygen. Detection of the content for MDA can reflect the level of lipid peroxidation in cells and reflect level of cellular damage indirectly.
Description
This kit can be used to measure the MDA content in animal culture cells and other samples.
Description
Biochemical
Assay Type
Biochemical kit
Detection Instrument
Microplate reader
Detection Method
Colorimetric method
Detection Range
0-113.0 nmol/ml
Experiment Instruments
Microplate reader
Inter-cv (%)
4.11
Intra-cv (%)
3.5
Manual
Recovery
104
Sensitivity
0.5nmol/ml
Target
MDA
Storage
Reagent 1: RT
Reagent 2: 4℃
Reagent 3: 4℃, shading light
Reagent 4: 4℃Reagent 5: 4℃
Valid Period
12 months, 12
Manual
Reviews of Malondialdehyde (MDA) Colorimetric Assay Kit (Cell Samples)