JC-1 Mitochondrial Membrane Potential Fluorescent Probe

JC-1 is an ideal fluorescent probe widely used for detecting mitochondrial membrane potential (??m). JC-1 dye accumulates in mitochondria in a potential-dependent manner, allowing for the detection of mitochondrial membrane potential in cells, tissues, or purified mitochondria. In healthy mitochondria, JC-1 aggregates in the mitochondrial matrix to form polymers that emit intense red fluorescence (Ex=585 nm, Em=590 nm); in unhealthy mitochondria, due to the decrease or loss of membrane potential, JC-1 exists only as a monomer in the cytoplasm, producing green fluorescence (Ex=514 nm, Em=529 nm). JC-1 is not only suitable for qualitative detection, as the color change can directly reflect the change in mitochondrial membrane potential, but also for quantitative detection, as the degree of mitochondrial depolarization can be measured by the ratio of red to green fluorescence intensity. For observation, simply use the conventional settings for observing red and green fluorescence.

The commonly used concentration range of JC-1 for detecting mitochondrial membrane potential in cells is 1–20 µg/mL, with 10 µg/mL being suitable for many cell types.

Features

Suitable for a variety of cell types.

Highly affinity to mitochondria.

Applications

This product can be used for detection of mitochondrial membrane potential in cells, tissues, or purified mitochondria 

Specifications

Components

Shipping and Storage

Transported with wet ice. Store the powder at -20°C in a dry, light-protected environment for at least one year. Aliquot the storage solution into single-use volumes and store at -20°C in a dry, light-protected environment to avoid repeated freeze-thaw cycles, which is effective for about one year.

Documents:

Safety Data Sheet

40705ES03-MSDS-CN20250114

40705ES08-MSDS-CN20250114

Manuals

40705_Manual_CN20250114_EN

Citations & References:

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