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Home / Assay Kits

Human Poly (ADP-ribose) Polymerase-1 - for 100 assays

BHE16300234

This product includes 17 ul of 0.7 mg/ml Human Poly (ADP-ribose) Polymerase-1

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Contact us to order
Tel
+1 866.986.9598
Email
orders@biohippo.com
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Product Manual Datasheet
Protocols Description Assay Protocol using the Human Poly (ADP-ribose) Polymerase-1 Assay Kit
1. Reagent preparation:
10 x DNA: dilute the 100 x DNA 10 –fold with water
10 x enzyme: dilute the 400 x Human PARP-1 40 –fold with 1 x Buffer
1 mM NAD+ : dilute the 10 mM stock 10 – fold with water
1 x fluorescence dye: dilute the 100 x fluorescence dye 100-fold with water
 
2. Reaction:
The total volume of each reaction mixture is 50 µl including: 30 µl of H2O, 5 µl of 10 x buffer, 5 µl of 10 x DNA, 5 µl of 10 x human PARP-1, 5 µl of 1 mM NAD+ . Incubate the reaction mixture at 37°C for 60 min.
 
3. Detection:
Add 200 µl of the 1 x fluorescence dye into the 50 µl of the reaction mixture. Measure the fluorescence intensity at 535 nm using the excitation wavelength at 485 nm.
Combination SKU Supplier No. Size Your price Quantity
BHE16300234-1 PARP-100 100 assays $421.56
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BHE16300234-2 PARP-1000 1000 assays $2314.81
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