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Home / Assay Kits

Human DNA polymerase beta assay kit (enzyme not included)

SKU : BHA16300003

This product includes all the assay reagents except the enzyme for 100 assays of human DNA polymerase beta reactions in a 96-well plate format.

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Mfr.No.	DPB100K
Description	DNA polymerase beta play key roles in the base excision repair (BER) process. It fills the small gaps (1 – 6 bases) of one DNA stand using the complementary strand as a template. Human DNA polymerase beta is an anti-cancer target. The human DNA polymerase beta assay is based on detection of the DNA polymerase activity in the presence of a DNA template and dNTPs. The Assay Kit (catalog number DPB100K) is to measure the activity of DNA polymerase beta and test DNA polymerase beta inhibitors. Each Human DNA Polymerase Beta Assay Kit (Catalog No: DPB100K) includes all the assay kit components except the enzyme for 100 assays in a 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of 100 x DNA template (3 µM), 33 µl of 100 x dNTP mix (10 mM), 1550 µl of 2 x Dye, 1550 µl of 50 mM EDTA.
Detection Method	/
Drug target assays	Anticancer drug targets

Product Manual	Datasheet
Protocols Description	Assay protocol The following assay protocol is based on the 384-well plate assay format (plate type: Matrix 4318 or alike). The reaction volume is 30 µl and the final assay volume is 60 µl. For 96-well plate assays (plate type: Costar 3915 or alike), the reaction volume is 50 µl and the final assay volume is 100 µl. 1. Reagent preparation: (1) 10 x DNA: Dilute the 100 x DNA 10-fold with water. Each assay uses 3 µl of 10 x DNA. (2) 10 x enzyme: Dilute the 100 x Human DNA polymerase beta 10-fold with the 1 x assay buffer. Each assay uses 3 µl of 10 x enzyme. (3) 10 x dNTP mix: Dilute the 100 x dNTP mix 10-fold with water. Each assay uses 3 µl of 10 x dNTP mix. (4) 1 x dye: Dilute the 2 x fluorescence dye 2-fold with 50 mM EDTA. Each assay uses 30 µl of 1 x dye. 2. Reaction: The total volume of each reaction mixture is 30 µl including 18 µl of H2O, 3 µl of 10 x Buffer, 3 µl of 10 x DNA template, 3 µl of 10 x enzyme, 3 µl of 10 x dNTP mix. Incubate the reaction mixture at 37°C for 60 min. 3. Detection: Mix 30 µl of the 1 x fluorescence dye with 30 µl of the reaction mixture. Measure the fluorescence intensity at 535 nm using the excitation wavelength at 485 nm.

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Combination SKU	Supplier No.	Specifications	Your price	Quantity
BHA16300003-1	DPB100K	enzyme not included	$365.40		Add to Cart
BHA16300003-2	DPB100KE	enzyme included	$613.14		Add to Cart

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