Matrices listed below were spiked with certain level of CCL4 and the recovery rates were calculated by comparing the measured value to the expected amount of CCL4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
95
EDTA plasma(n=5)
89-105
94
heparin plasma(n=5)
93-103
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CCL4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
CCL4 ELISA Kit allows for the in vitro quantitative determination of CCL4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human CCL4(C-C motif chemokine 4) ELISA Kit