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Home / Assay Kits

Glucose-6-phosphate (G6P) Colorimetric Assay Kit

SKU : BHE10308181

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Elabscience Biotechnology Co.,Ltd

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Mfr.No.	E-BC-K011-M
Assay Time	35 min
Assay Type	QuantitatIVe
Inter-cv (%)	4.3
Intra-cv (%)	2.1
Manual	Data Sheet
Recovery	95
Sensitivity	5.6 umol /L
Test Principle	Glucose-6-phosphate (G6P) is a molecule generated by phosphorylation of hydroXyl groups on the siXth carbon of glucose under the catalysis of heXokinase. It is a common small molecule of sugar metabolism in cells and participates in biochemical pathways such as glycolysis and pentose phosphate pathway. In the first reaction of glycolysis, glucose is catalyzed by heXokinase to produce glucose-6-phosphate, which is then catalyzed by phosphoglucose isomerase to form fructose-6-phosphate to continue the other steps of glycolysis: In the pentose phosphate pathway, glucose-6-phosphate is the first substrate, and this process is also the main way to generate NADPH. In addition to these two metabolic pathways, glucose-6-phosphate can also be converted into glycogen or starch and stored.

Research Area	Glycolysis & Carbohydrates

Dilutions	It isrecommended to take 2~3 samples with expected large difference to dopre-experiment before formal experiment and dilute the sample according to theresult of the pre-experiment and the detection range ( 0.09-40ug/mL ).The recommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor, Human serum Human plasma 10% Mouse liver tissue homogenization Rat serum Rat plasma 10% Carrot tissue homogenization 1. Note:Thediluentisnormalsaline(0.9%NaCl)orPBS(0.01M,pH7.4).
Storage	-20°C
Valid Period	6 Months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10308181	E-BC-K011-M	96T	$360		Add to Cart

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