Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate for 20 minute incubation: 0.03 to 25 U/L xanthine oxidase for colorimetric assays and 0.01 to 2.5 U/L for fluorimetric assays.Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature.
Intended Use
OD570nm,FL530/585nm
Detection Principle
FL 0.01 U/L; OD 0.03 U/L
Shelf Life
6 months
Specifity
For quantitative determination of xanthine oxidase enzyme activity.
Reference
El-Shennawy, L et al. (2020). Dose-dependent reproductive toxicity of sodium benzoate in male rats: Inflammation, oxidative stress and apoptosis. Reproductive Toxicology, 98: 92-98. Assay: Xanthine Oxidase in rat testes.
Sharma, S et al. (2020). Ameliorative effect of a standardized polyherbal combination in methotrexate-induced nephrotoxicity in the rat. Pharmaceutical Biology, 58(1): 184-199. Assay: Xanthine Oxidase in plant extract inhition.
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