Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate for 30 minute incubation: 0.01 to 2.5 mM xanthine for colorimetric assays and 3 to 250 ?M for fluorimetric assays. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature.Fast and high-throughput. Assays using 96-well plates and liquid handling system could allow simultaneous processing tens of thousands of samples per day.
Intended Use
OD570nm,FL530/585nm
Detection Principle
FL 3 µM; OD 10 µM
Shelf Life
6 months
Specifity
For quantitative determination of xanthine and drug effects on its metabolism.