Sensitive and accurate. Use as little as 10 uL samples. Linear detection range in 96-well plate: 0.02 to 1 mM sialic acid for colorimetric assays and 2 to 100 uM for fluorimetric assays.Simple and convenient. Can detect free sialic acid by addition of a single working reagent and incubation for 60 min at room temperature or total sialic acid by pre-treating samples with a 60 min hydrolysis step.
Intended Use
OD570nm,or FL530/585nm
Detection Principle
OD, FL: 20, 2 uM
Shelf Life
6 months
Specifity
For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on its metabolism.
Reference
Cadaoas, J., et al. (2021). Galactosialidosis: Preclinical enzyme replacement therapy in a mouse model of the disease, a proof of concept. Molecular Therapy - Methods & Clinical Development. 20: 191-203. Assay: Sialic acid in mouse urine.
Ha, T. K., et al. (2020). Knockout of sialidase and pro-apoptotic genes in Chinese hamster ovary cells enables the production of recombinant human erythropoietin in fed-batch cultures. Metabolic Engineering. 57: 182-192. Assay: Sialic acid in hamster cell line recombinant erythropoietin.
Mosca, R., et al. (2020). Conventional and unconventional therapeutic strategies for sialidosis type I. Journal of Clinical Medicine. 9(3): 695. Assay: Sialic acid in mouse urine.
Odaka, H., et al. (2021). An iPSC-based neural model of sialidosis uncovers glycolytic impairment-causing presynaptic dysfunction and deregulation of Ca2+ dynamics. Neurobiology of Disease. 152: 105279. Assay: Sialic acid in human fibroblast.
Chrostek, L et al (2014). Serum Sialic Acids Levels According to the Severity of Liver Cirrhosis. Journal of clinical laboratory analysis. Assay: Sialic acid in human serum.
Chrostek, L et al (2014). Sialic acid level reflects the disturbances of glycosylation and acute-phase reaction in rheumatic diseases. Rheumatol International. 34(3):393-9. Assay: Sialic acid in human serum.
Ganther, M et al (2014). Partial synthesis of ganglioside and lysoganglioside lipoforms as internal standards for MS quantification. J Lipid Res. 55(12):2692-704. Assay: Sialic acid in bovine brain gangliosides.
Ha, TK, Kim, YG, Lee, GM (2014). Effect of lithium chloride on the production and sialylationof Fc-fusion protein in Chinese hamster ovary cell culture. Appl Microbiol Biotechnol. 98(22):9239-48. Assay: Sialic acid in hamster ovary cells.
Bonten, EJ et al (2013). Chaperone-mediated gene therapy with recombinant AAV-PPCA in a new mouse model of type I sialidosis. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease 1832.10: 1784-1792. Assay: Sialic acid in mouse kidney tissue.
Park, KH et al (2013). Structural and biochemical characterization of the broad substrate specificity of Bacteroides thetaiotaomicron commensal sialidase. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics1834.8: 1510-1519. Assay: Sialic acid in B. thetaiotaomicron cells.
Sarangarajan, R et al(2011). Methods for treatment of a sarcoma using an epimetabolic shifter (CoEnzyme Q10). US Patent Appl. US 2011/0064747 A1. Assay: Sialic Acid in lipoproteins.
Cylwik B et al (2010). The changes of sialic acid concentration and content in apolipoprotein B-containing lipoproteins in the sera of alcoholics. Alcohol Alcohol. 45(5):422-6. Assay: Sialic acid in human serum.
Cylwik B, et al (2010). The changes of sialic acid concentration and content in apolipoprotein B-containing lipoproteins in the sera of alcoholics. Alcohol Alcohol. 45(5):422-6. Assay: Sialic acid in lipoproteins.
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