Sensitive. Use 10 uL samples. Detection range: colorimetric assay 0.06 - 10 U/L, fluorimetric assay 0.04 - 1 U/L.Simple and High-throughput: the assay involves addition of a single working reagent and can be readily adapted to high-throughput assays for drug screening.
Intended Use
OD570nm,or FL530/585nm
Detection Principle
0.04 U/L
Shelf Life
6 months
Specifity
For quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.
Reference
Zhu X et al (2014). The role of phospholipase D1 in liver fibrosis induced by dimethylnitrosamine in vivo. Dig Dis Sci. 59(8):1779-88. Assay: Phospholipase D in rat tissue.
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