Fast and sensitive. Linear detection range: 4 to 400 uM citrate for colorimetric assays and 0.5 to 40 uM for fluorimetric assays.Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Intended Use
OD570nm,or FL530/585nm
Detection Principle
OD, FL: 4, 0.5 uM.
Shelf Life
6 months
Specifity
For quantitative determination of citrate or citric acid and evaluation of drug effects on citrate metabolism.
Reference
Pant, A. et al (2021). Viral growth factor-and STAT3 signaling-dependent elevation of the TCA cycle intermediate levels during vaccinia virus infection. PLoS Pathogens, 17(2). Assay: Citrate in human foreskin fibroblasts.
Fu, Xuekun, et al (2018). Runx2/Osterix and zinc uptake synergize to orchestrate osteogenic differentiation and citrate containing bone apatite formation. Advanced Science 5.4: 1700755. Assay: Citrate in mice cells.
Trivedi, Amit Kumar, et al. (2015). Adaptation of oxidative phosphorylation to photoperiod-induced seasonal metabolic states in migratory songbirds. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 184): 34-40. Assay: Citrate in bird plasma.
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