Sensitive and accurate. Linear detection range in 96-well plate: 0.1 to 10 mg/dL cholesterol for colorimetric assays and 0.02 to 2 mg/dL for fluorimetric assays.Convenient. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Intended Use
OD570nm,or FL530/585nm
Detection Principle
OD, FL: 1, 0.2 mg/dL
Shelf Life
12 months
Specifity
For quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism.
Reference
Hernandez-Corroto, E. et al. (2020). Sustainable extraction of proteins and bioactive substances from pomegranate peel (Punica granatum L.) using pressurized liquids and deep eutectic solvents.Innovative Food Science & Emerging Technologies. 60: 102314. Assay: Cholesterol in pomegranate peel extracts.
Wilson, L. J. (2020). Integrating upstream and downstream process development strategies for mammalian cell derived therapeutic antibodies (Master's thesis, UCL (University College London), 2020). 1-227. Assay: Cholesterol in hamster cells.
Attah, F. A., Asaleye, C. M., Omisore, A. D., Kolawole, B. A., Aderibigbe, A. S., & Alo, M. (2019). Relationship between sonographically measured median nerve cross-sectional area and presence of peripheral neuropathy in diabetic subjects. World journal of diabetes, 10(1), 47. Assay: Cholesterol in human blood.
Kumar, A., Pandita, S., Anand Laxmi, N., Bhakat, M., & Mohanty, T. K. (2018). Effects of prostasomes on functional parameters of fresh and cryopreserved-thawed spermatozoa of crossbred Karan Fries (KF) bulls. Indian. J Anim. Res. Assay: Cholesterol in bulls plasma.
Chappuis, E., Morel-Depeisse, F., Bariohay, B., & Roux, J. (2017). Alpha-galacto-oligosaccharides at low dose improve liver steatosis in a high-fat diet mouse model. Molecules, 22(10), 1725. Assay: Cholesterol in mice plasma.
Gallagher, A. J., Skubel, R. A., Pethybridge, H. R., & Hammerschlag, N. (2017). Energy metabolism in mobile, wild-sampled sharks inferred by plasma lipids. Conservation physiology 5(1):cox002. Assay: Cholesterol in shark blood.
Sinha, P. B., Tesfaye, D., Rings, F., Hossien, M., Hoelker, M., Held, E. & Salilew-Wondim, D. (2017). MicroRNA-130b is involved in bovine granulosa and cumulus cells function, oocyte maturation and blastocyst formation. Journal of ovarian research, 10(1), 37. Assay: Cholesterol in bovine cells.
Raij, L., Tian, R., Wong, J. S., He, J. C., & Campbell, K. N. (2016). Podocyte injury: the role of proteinuria, urinary plasminogen, and oxidative stress. American Journal of Physiology-Renal Physiology, 311(6), F1308-F1317. Assay: Cholesterol in human podocyte.
Guevara-Arauza, J.C., et al. (2011). Biofunctional activity of tortillas and bars enhanced with nopal. Preliminary assessment of functional effect after intake on the oxidative status in healthy volunteers. Chem Cent J 5(1):10. Assay: Cholesterol in human plasma.
Stoll, C., et al. (2011). Liposomes alter thermal phase behavior and composition of red blood cell membranes. Biochim Biophys Acta 1808(1):474-81. Assay: Cholesterol in human red blood cells.
Uddin, M.J., et al. (2011). Detection of quantitative trait loci affecting serum cholesterol, LDL, HDL, and triglyceride in pigs. BMC Genet 12:62. Assay: Cholesterol in pig serum.
Ponda, MP et al (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis 210(1):57-62. Assay: Cholesterol in mouse blood.
Rub A et al (2009). Cholesterol depletion associated with Leishmania major infection alters macrophage CD40 signalosome composition and effector function. Nat Immunol. 10(3):273-80. Assay: Cholesterol in mouse macrophage, membrane.
Lee, SM et al (2008).GCG-rich tea catechins are effective in lowering cholesterol and triglyceride concentrations in hyperlipidemic rats. Lipids 43(5): 419-429. Assay: cholesterol in rat blood.
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