ELISA Kit for Rat Glutathione peroxidase 3

Mfr.No.

E9949r

Alternative Names

Plasma glutathione peroxidase ; GPx-P

Categories

Elisa

Function

Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.

Specificity

Natural and recombinant Rat Glutathione peroxidase 3

Subcellular Location

Secreted

Detection Range

0.78-50ng/mL

Entryname

GPX3_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-101%	92-103%	92-105%	94-106%
EDTA plasma(n=5)	89-97%	93-102%	92-98%	90-103%
heparin plasma(n=5)	93-101%	94-104%	101-108%	91-104%

Method Type

Sandwich

Platform

PF00255

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 5.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.8%

Protein Name

Glutathione peroxidase 3

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	91-104
Plasma	95	92-103

Sensitivity

0.419ng/mL

Sequence

50:

MARILRASCL ; LSLLLAGFVP ; PGRGQEKSKT ; DCHGGMSGTI ; YEYGALTIDG ;

100:

EEYIPFKQYA ; GKYILFVNVA ; SYUGLTDQYL ; ELNALQEELG ; PFGLVILGFP ;

150:

CNQFGKQEPG ; ENSEILPSLK ; YVRPGGGFVP ; NFQLFEKGDV ; NGEKEQKFYT ;

200:

FLKNSCPPTA ; ELLGSPGRLF ; WEPMKIHDIR ; WNFEKFLVGP ; DGIPIMRWYH ;

226:

RTTVSNVKMD ; ILSYMRRQAA ; LGARGK

Sequence Length

226

String

P23764

Sub Unit

Homotetramer.

Uniprot	P23764
Gene Name	Gpx3
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:64317
Unigene	Rn.108074

Reference	1. "Tissue specific expression of the plasma glutathione peroxidase gene in rat kidney." Yoshimura S., Watanabe K., Suemizu H., Onozawa T., Mizoguchi J., Tsuda K., Hatta H., Moriuchi T. J. Biochem.109:918-923(1991) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Kidney. 2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] tissue: Pituitary.

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE13803565	E9949r	96T	$796		Add to Cart

ELISA Kit for Rat Glutathione peroxidase 3

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of ELISA Kit for Rat Glutathione peroxidase 3

Rate it now..!!