ELISA Kit for Rat Cytochrome c, somatic

Mfr.No.

E0594r

Categories

Elisa

Function

Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

Specificity

Natural and recombinant Rat Cytochrome c, somatic

Subcellular Location

Mitochondrion matrix

Detection Range

0.312-20ng/mL

Entryname

CYC_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

Method Type

Sandwich

Platform

PF00034

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

Protein Name

Cytochrome c, somatic

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

Sensitivity

0.167ng/mL

Sequence

50:

MGDVEKGKKI ; FVQKCAQCHT ; VEKGGKHKTG ; PNLHGLFGRK ; TGQAAGFSYT ;

100:

DANKNKGITW ; GEDTLMEYLE ; NPKKYIPGTK ; MIFAGIKKKG ; ERADLIAYLK ;

105:

KATNE

Sequence Length

105

String

P62898

Uniprot	P62898
Gene Name	Cycs
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:100363502
Unigene	Rn.161841

Reference	1. "Isolation and structure of a rat cytochrome c gene." Scarpulla R.C., Agne K.M., Wu R. J. Biol. Chem.256:6480-6486(1981) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] 2. "Nonallelic members of the cytochrome c multigene family of the rat may arise through different messenger RNAs." Scarpulla R.C., Wu R. Cell32:473-482(1983) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] 3. "Structure and expression of rodent genes encoding the testis-specific cytochrome c. Differences in gene structure and evolution between somatic and testicular variants." Virbasius J.V., Scarpulla R.C. J. Biol. Chem.263:6791-6796(1988) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] tissue: Heart. tissue: Ovary. 5. "Primary structure of mouse, rat, and guinea pig cytochrome c." Carlson S.S., Mross G.A., Wilson A.C., Mead R.T., Wolin L.D., Bowers S.F., Foley N.T., Muijsers A.O., Margoliash E. Biochemistry16:1437-1442(1977) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-105;ACETYLATION AT GLY-2 6. Lubec G., Kang S.U. Submitted (2007-07) to the UniProtKB [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 40-54; 57-73 AND 93-100;IDENTIFICATION BY MASS SPECTROMETRY strain: Sprague-Dawley. tissue: Brain.

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ELISA Kit for Rat Cytochrome c, somatic

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