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ELISA Kit for Rat Calreticulin

SKU : BHE13801904

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E1486r
Alternative Names	CALBP ; CRP55 ; Calcium-binding protein 3 ; CABP3 ; Calregulin ; Endoplasmic reticulum resident protein 60 ; ERp60 ; HACBP
Categories	Elisa
Function	Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
Specificity	Natural and recombinant Rat Calreticulin
Subcellular Location	Endoplasmic reticulum lumen
Detection Range	0.39-25ng/mL
Entryname	CALR_RAT
Method Type	Sandwich
Platform	PF00262
Protein Name	Calreticulin
Sensitivity	0.121ng/mL
Sequence	50: MLLSVPLLLG ; LLGLAAADPA ; IYFKEQFLDG ; DAWTNRWVES ; KHKSDFGKFV ; 100: LSSGKFYGDQ ; EKDKGLQTSQ ; DARFYALSAR ; FEPFSNKGQT ; LVVQFTVKHE ; 150: QNIDCGGGYV ; KLFPGGLDQK ; DMHGDSEYNI ; MFGPDICGPG ; TKKVHVIFNY ; 200: KGKNVLINKD ; IRCKDDEFTH ; LYTLIVRPDN ; TYEVKIDNSQ ; VESGSLEDDW ; 250: DFLPPKKIKD ; PDAAKPEDWD ; ERAKIDDPTD ; SKPEDWDKPE ; HIPDPDAKKP ; 300: EDWDEEMDGE ; WEPPVIQNPE ; YKGEWKPRQI ; DNPDYKGTWI ; HPEIDNPEYS ; 350: PDANIYAYDS ; FAVLGLDLWQ ; VKSGTIFDNF ; LITNDEAYAE ; EFGNETWGVT ; 400: KAAEKQMKDK ; QDEEQRLKEE ; EEDKKRKEEE ; EAEDKEDEDD ; RDEDEDEEDE ; 416: KEEDEEDATG ; QAKDEL
Sequence Length	416
String	P18418
Sub Unit	Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with GABARAP, NR3C1 and TRIM21 (By similarity). Interacts with PDIA3/ERp57.

Uniprot	P18418
Gene Name	Calr
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:64202
Unigene	Rn.974

Reference	1. "Structural homology between the rat calreticulin gene product and the Onchocerca volvulus antigen Ral-1." Murthy K.K., Banville D., Srikant C.B., Carrier F., Bell A., Holmes C., Patel Y.C. Nucleic Acids Res.18:4933-4933(1990) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: Sprague-Dawley. tissue: Brain cortex. 2. "An endoplasmic reticulum protein, calreticulin, is transported into the acrosome of rat sperm." Nakamura M., Moriya M., Baba T., Michikawa Y., Yamanobe T., Arai K., Okinaga S., Kobayashi T. Exp. Cell Res.205:101-110(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: Sprague-Dawley. 3. "Retention and retrieval: both mechanisms cooperate to maintain calreticulin in the endoplasmic reticulum." Soennichsen B., Fuellekrug J., van Nguyen P., Diekmann W., Robinson D.G., Mieskes G. J. Cell Sci.107:2705-2717(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: Sprague-Dawley. tissue: Liver. 4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] tissue: Prostate. 5. "Calreticulin is present in the acrosome of spermatids of rat testis." Nakamura M., Michikawa Y., Baba T., Okinaga S., Arai K. Biochem. Biophys. Res. Commun.186:668-673(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 18-32 strain: Sprague-Dawley. tissue: Testis. 6. "Identification of protein disulfide isomerase and calreticulin as autoimmune antigens in LEC strain of rats." Yokoi T., Nagayama S., Kajiwara R., Kawaguchi Y., Horiuchi R., Kamataki T. Biochim. Biophys. Acta1158:339-344(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 18-32 strain: LEC. tissue: Liver. 7. "Calreticulin is a candidate for a calsequestrin-like function in Ca2(+)-storage compartments (calciosomes) of liver and brain." Treves S., de Mattei M., Lanfredi M., Villa A., Green N.M., Maclennan D.H., Meldolesi J., Pozzan T. Biochem. J.271:473-480(1990) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 18-29 8. Lubec G., Afjehi-Sadat L., Diao W. Submitted (2007-04) to the UniProtKB [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 25-36; 88-98 AND 163-185;IDENTIFICATION BY MASS SPECTROMETRY strain: Sprague-Dawley. tissue: Hippocampus. tissue: Spinal cord. 9. Lone Y.-C., Bailly A., Latruffe N. Submitted (1988-12) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 269-361 strain: Sprague-Dawley. 10. "Calreticulin -- an endoplasmic reticulum protein with calcium-binding activity is also found in the extracellular matrix." Somogyi E., Petersson U., Hultenby K., Wendel M. Matrix Biol.22:179-191(2003) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY;SUBCELLULAR LOCATION 11. "TROSY-NMR reveals interaction between ERp57 and the tip of the calreticulin P-domain." Frickel E.M., Riek R., Jelesarov I., Helenius A., Wuethrich K., Ellgaard L. Proc. Natl. Acad. Sci. U.S.A.99:1954-1959(2002) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PDIA3 12. "NMR structure of the calreticulin P-domain." Ellgaard L., Riek R., Herrmann T., Guntert P., Braun D., Helenius A., Wuthrich K. Proc. Natl. Acad. Sci. U.S.A.98:3133-3138(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 206-305 13. "NMR structures of 36 and 73-residue fragments of the calreticulin P-domain." Ellgaard L., Bettendorff P., Braun D., Herrmann T., Fiorito F., Jelesarov I., Guntert P., Helenius A., Wuthrich K. J. Mol. Biol.322:773-784(2002) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 206-278

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