ELISA Kit for Mouse Transcription factor SOX-2

Mfr.No.

E0423m

Alternative Names

Sox-2

Categories

Elisa

Function

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation.

Specificity

Natural and recombinant Mouse Transcription factor SOX-2

Subcellular Location

Nucleus

Detection Range

0.156-10ng/mL

Entryname

SOX2_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SOX2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-103%	92-102%	89-106%	93-100%
EDTA plasma(n=5)	92-104%	90-107%	95-101%	94-104%
heparin plasma(n=5)	88-107%	94-103%	93-102%	91-106%

Method Type

Sandwich

Platform

PF00505

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

Protein Name

Transcription factor SOX-2

Recovery

Recovery was determined by spiking various levels of SOX2 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-104
Plasma	98	94-107

Sensitivity

0.058ng/mL

Sequence

50:

MYNMMETELK ; PPGPQQASGG ; GGGGGNATAA ; ATGGNQKNSP ; DRVKRPMNAF ;

100:

MVWSRGQRRK ; MAQENPKMHN ; SEISKRLGAE ; WKLLSETEKR ; PFIDEAKRLR ;

150:

ALHMKEHPDY ; KYRPRRKTKT ; LMKKDKYTLP ; GGLLAPGGNS ; MASGVGVGAG ;

200:

LGGGLNQRMD ; SYAHMNGWSN ; GSYSMMQEQL ; GYPQHPGLNA ; HGAAQMQPMH ;

250:

RYVVSALQYN ; SMTSSQTYMN ; GSPTYSMSYS ; QQGTPGMALG ; SMGSVVKSEA ;

300:

SSSPPVVTSS ; SHSRAPCQAG ; DLRDMISMYL ; PGAEVPEPAA ; PSRLHMAQHY ;

319:

QSGPVPGTAK ; YGTLPLSHM

Sequence Length

319

String

P48432

Sub Unit

Interacts with ZSCAN10. Interacts with SOX3 and FGFR1.

Uniprot	P48432
Gene Name	Sox2
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:20674
Unigene	Mm.65396

Reference	1. "Developmental-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3." Yuan H., Corbi N., Basilico C., Dailey L. Genes Dev.9:2635-2645(1995) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 2. Yuan H., Corbi N., Basilico C., Dailey L. Submitted (1998-08) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SEQUENCE REVISION 3. "A comparison of the properties of Sox-3 with Sry and two related genes, Sox-1 and Sox-2." Collignon J., Sockanathan S., Hacker A., Cohen-Tannoudji M., Norris D., Rastan S., Stevanovic M., Goodfellow P.N., Lovell-Badge R. Development122:509-520(1996) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] strain: 129. 4. "Inhibition of DNA binding of Sox2 by the SUMO conjugation." Tsuruzoe S., Ishihara K., Uchimura Y., Watanabe S., Sekita Y., Aoto T., Saitoh H., Yuasa Y., Niwa H., Kawasuji M., Baba H., Nakao M. Biochem. Biophys. Res. Commun.351:920-926(2006) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUMOYLATION AT LYS-247;MUTAGENESIS OF LYS-247;SUBCELLULAR LOCATION;FUNCTION 5. "Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors." Takahashi K., Yamanaka S. Cell126:663-676(2006) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: BIOTECHNOLOGY 6. "SOX3 activity during pharyngeal segmentation is required for craniofacial morphogenesis." Rizzoti K., Lovell-Badge R. Development134:3437-3448(2007) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SOX3 AND FGFR1 7. "Zfp206, Oct4, and Sox2 are integrated components of a transcriptional regulatory network in embryonic stem cells." Yu H.B., Kunarso G., Hong F.H., Stanton L.W. J. Biol. Chem.284:31327-31335(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ZSCAN10 8. "Ars2 maintains neural stem-cell identity through direct transcriptional activation of Sox2." Andreu-Agullo C., Maurin T., Thompson C.B., Lai E.C. Nature481:195-198(2012) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INDUCTION

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ELISA Kit for Mouse Transcription factor SOX-2

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