ELISA Kit for Mouse Sex-determining region Y protein

Mfr.No.

E8883m

Alternative Names

Testis-determining factor ; Tdf ; Tdy

Categories

Elisa

Function

Transcriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. In male adult brain involved in the maintenance of motor functions of dopaminergic neurons (By similarity). Involved in different aspects of gene regulation including promoter activation or repression. SRY HMG box recognizes DNA by partial intercalation in the minor groove. Promotes DNA bending. Also involved in pre-mRNA splicing (By similarity). Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3'.

Specificity

Natural and recombinant Mouse Sex-determining region Y protein

Subcellular Location

Nucleus speckle Cytoplasm Colocalizes in the nucleus with ZNF208 isoform KRAB-O and tyrosine hydroxylase (TH). Colocalizes with SOX6 in speckles (By similarity). Colocalizes with CAML in the nucleus.

Entryname

SRY_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SRY and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-103%	91-104%	90-107%	92-106%
EDTA plasma(n=5)	90-108%	95-102%	92-106%	94-105%
heparin plasma(n=5)	94-103%	90-107%	93-103%	91-108%

Method Type

Sandwich

Platform

PF00505

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.7%

Protein Name

Sex-determining region Y protein

Recovery

Recovery was determined by spiking various levels of SRY into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	98	93-104
Plasma	99	92-105

Sequence

50:

MEGHVKRPMN ; AFMVWSRGER ; HKLAQQNPSM ; QNTEISKQLG ; CRWKSLTEAE ;

100:

KRPFFQEAQR ; LKILHREKYP ; NYKYQPHRRA ; KVSQRSGILQ ; PAVASTKLYN ;

150:

LLQWDRNPHA ; ITYRQDWSRA ; AHLYSKNQQS ; FYWQPVDIPT ; GHLQQQQQQQ ;

200:

QQQQFHNHHQ ; QQQQFYDHHQ ; QQQQQQQQQQ ; QFHDHHQQKQ ; QFHDHHQQQQ ;

250:

QFHDHHHHHQ ; EQQFHDHHQQ ; QQQFHDHQQQ ; QQQQQQQQFH ; DHHQQKQQFH ;

300:

DHHHHQQQQQ ; FHDHQQQQQQ ; FHDHQQQQHQ ; FHDHPQQKQQ ; FHDHPQQQQQ ;

350:

FHDHHHQQQQ ; KQQFHDHHQQ ; KQQFHDHHQQ ; KQQFHDHHQQ ; QQQFHDHHQQ ;

395:

QQQQQQQQQQ ; QFHDQQLTYL ; LTADITGEHT ; PYQEHLSTAL ; WLAVS

Sequence Length

395

String

Q05738

Sub Unit

Interacts with KPNB1, ZNF208 isoform KRAB-O, PARP1 and SLC9A3R2. The interaction with KPNB1 is sensitive to dissociation by Ran in the GTP-bound form. Interaction with PARP1 impaired its DNA-binding activity. Interacts with CALM, EP300, HDAC3 and WT1 (By similarity). The interaction with EP300 modulates its DNA-binding activity.

Uniprot	Q05738
Gene Name	Sry
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:21674
Unigene	Mm.377114

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BHE13810354	E8883m	96T	$776		Add to Cart

ELISA Kit for Mouse Sex-determining region Y protein

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