ELISA Kit for Mouse Protransforming growth factor alpha

Mfr.No.

E0123m

Categories

Elisa

Function

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

Specificity

Natural and recombinant Mouse Protransforming growth factor alpha

Subcellular Location

Protransforming growth factor alpha Cell membrane Single-pass type I membrane protein

Detection Range

15.6-1000pg/mL

Entryname

TGFA_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TGFA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-100%	96-103%	94-99%	94-101%
EDTA plasma(n=5)	96-105%	90-98%	92-101%	93-104%
heparin plasma(n=5)	92-101%	95-99%	97-102%	96-103%

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.1%

Protein Name

Protransforming growth factor alpha

Recovery

Recovery was determined by spiking various levels of TGFA into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	98	95-102
Plasma	97	93-104

Sensitivity

7.81pg/mL

Sequence

50:

MVPATGQLAL ; LALGILLAVC ; QALENSTSPL ; SDSPVAAAVV ; SHFNKCPDSH ;

100:

TQYCFHGTCR ; FLVQEEKPAC ; VCHSGYVGVR ; CEHADLLAVV ; AASQKKQAIT ;

150:

ALVVVSIVAL ; AVLIITCVLI ; HCCQLRKHCE ; WCRALVCRHE ; KPSALLKGRT ;

159:

ACCHSETVV

Sequence Length

159

String

P48030

Sub Unit

Interacts with the PDZ domains of SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2 (By similarity). Interacts with MAGI3.

Uniprot	P48030
Gene Name	Tgfa
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:21802
Unigene	Mm.137222

Reference	1. "Nucleotide sequence and tissue distribution of mouse transforming growth factor-alpha." Vaughan T.J., Pascall J.C., Brown K.D. Biochim. Biophys. Acta1132:322-324(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 2. "Characterization of the mouse transforming growth factor alpha gene: its expression during eyelid development and in waved 1 tissues." Berkowitz E.A., Seroogy K.B., Schroeder J.A., Russell W.E., Evans E.P., Riedel R.F., Phillips H.K., Harrison C.A., Lee D.C., Luetteke N.C. Cell Growth Differ.7:1271-1282(1996) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] strain: 129/Sv. tissue: Brain. 3. "Identification of MAGI-3 as a transforming growth factor-alpha tail binding protein." Franklin J.L., Yoshiura K., Dempsey P.J., Bogatcheva G., Jeyakumar L., Meise K.S., Pearsall R.S., Threadgill D., Coffey R.J. Exp. Cell Res.303:457-470(2005) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH MAGI3

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BHE13811312	E0123m	96T	$776		Add to Cart

ELISA Kit for Mouse Protransforming growth factor alpha

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