ELISA Kit for Mouse Melanocyte protein PMEL

Mfr.No.

E2299m

Alternative Names

Melanocyte protein Pmel 17 ; Premelanosome protein ; Silver locus protein ; D10H12S53E ; Pmel17 ; Si ; Silv

Categories

Elisa

Function

Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures.

Specificity

Natural and recombinant Mouse Melanocyte protein PMEL

Subcellular Location

M-alpha Secreted

Detection Range

78-5000pg/ml

Entryname

PMEL_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PMEL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	104-110%	102-113%	100-116%	104-114%
EDTA plasma(n=5)	105-110%	98-116%	104-112%	102-112%
heparin plasma(n=5)	100-114%	103-112%	100-115%	101-113%

Method Type

Sandwich

Platform

PF00801

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.1%

Protein Name

Melanocyte protein PMEL

Recovery

Recovery was determined by spiking various levels of PMEL into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	108	105-113
Plasma	107	104-112

Sensitivity

39.3pg/mL

Sequence

50:

MVGVQRRSFL ; PVLVLSALLA ; VGALEGSRNQ ; DWLGVPRQLV ; TKTWNRQLYP ;

100:

EWTEVQGSNC ; WRGGQVSLRV ; INDGPTLVGA ; NASFSIALHF ; PGSQKVLPDG ;

150:

QVIWANNTII ; NGSQVWGGQP ; VYPQEPDDAC ; VFPDGGPCPS ; GPKPPKRSFV ;

200:

YVWKTWGKYW ; QVLGGPVSRS ; SIATRHAKLG ; THTMEVTVYH ; RRGSQSYVPL ;

250:

AHASSTFTIT ; DQVPFSVSVS ; QLQALDGETK ; HFLRNHPLIF ; ALQLHDPSGY ;

300:

LAEADLSYTW ; DFGDGTGTLI ; SRALDVTHTY ; LESGSVTAQV ; VLQAAIPLVS ;

350:

CGSSPVPGTT ; DGYMPTAEAP ; GTTSRQGTTT ; KVVGTTPGQM ; PTTQPSGTTV ;

400:

VQMPTTEVTA ; TTSEQMLTSA ; VIDTTLAEVS ; TTEGTGTTPT ; RPSGTTVAQA ;

450:

TTTEGPDASP ; LLPTQSSTGS ; ISPLLDDTDT ; IMLVKRQVPL ; DCVLYRYGSF ;

500:

SLALDIVQGI ; ESAEILQAVP ; FSEGDAFELT ; VSCQGGLPKE ; ACMDISSPGC ;

550:

QPPAQRLCQS ; VPPSPDCQLV ; LHQVLKGGSG ; TYCLNVSLAD ; ANSLAVASTQ ;

600:

LVVPGQDGGL ; GQAPLLVGIL ; LVLVAVVLAS ; LILGIDLRSR ; AQFPKCHMVA ;

626:

LTAAPASGLR ; ARGLGENSPL ; LSGQQV

Sequence Length

626

String

Q60696

Sub Unit

Heterooligomer; disulfide-linked heterooligomers of M-alpha and M-beta. Interacts with MLANA.

Uniprot	Q60696
Gene Name	Pmel
Immunogen Species	Mus musculus (Mouse)
Unigene	Mm.16756

Reference	1. "Mouse silver mutation is caused by a single base insertion in the putative cytoplasmic domain of Pmel 17." Kwon B.S., Halaban R., Ponnazhagan S., Kim K., Chintamaneni C., Bennett D., Pickard R.T. Nucleic Acids Res.23:154-158(1995) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANTS SILVER strain: C57BL/6. tissue: Skin. 2. "Pmel17 initiates premelanosome morphogenesis within multivesicular bodies." Berson J.F., Harper D.C., Tenza D., Raposo G., Marks M.S. Mol. Biol. Cell12:3451-3464(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEOLYTIC PROCESSING

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ELISA Kit for Mouse Melanocyte protein PMEL

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