ELISA Kit for Mouse Histone H4

Mfr.No.

E1485m

Categories

Elisa

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Specificity

Natural and recombinant Mouse Histone H4

Subcellular Location

Nucleus Chromosome

Detection Range

0.156-10ng/mL

Entryname

H4_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	87-99%	88-101%	90-103%	89-101%
EDTA plasma(n=5)	88-99%	90-104%	92-102%	95-108%
heparin plasma(n=5)	90-102%	93-104%	95-106%	93-103%

Method Type

Sandwich

Platform

PF00125

Precision

Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 8.1%

Protein Name

Histone H4

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	89	88-102
Plasma	90	89-101

Sensitivity

0.093ng/mL

Sequence

50:

MSGRGKGGKG ; LGKGGAKRHR ; KVLRDNIQGI ; TKPAIRRLAR ; RGGVKRISGL ;

100:

IYEETRGVLK ; VFLENVIRDA ; VTYTEHAKRK ; TVTAMDVVYA ; LKRQGRTLYG ;

103:

FGG

Sequence Length

103

String

P62806

Sub Unit

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

Uniprot	P62806
Gene Name	Hist1h4a
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:100041230
Unigene	Mm.14775

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SKU	Supplier No.	Size	Your price	Quantity
BHE13805725	E1485m	96T	$776		Add to Cart

ELISA Kit for Mouse Histone H4

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