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Home / Assay Kits / ELISA Kits

ELISA Kit for Mouse E3 ubiquitin-protein ligase SMURF2

SKU : BHE13810207

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E2373m
Alternative Names	HECT-type E3 ubiquitin transferase SMURF2 ; SMAD ubiquitination regulatory factor 2 ; SMAD-specific E3 ubiquitin-protein ligase 2
Categories	Elisa
Function	E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
Specificity	Natural and recombinant Mouse E3 ubiquitin-protein ligase SMURF2
Subcellular Location	Nucleus Cytoplasm Cell membrane Membrane raft Cytoplasmic in the presence of SMAD7 (By similarity). Co-localizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts (By similarity). Interacts with STAMBP and RNF11.
Entryname	SMUF2_MOUSE
Platform	PF00168
Protein Name	E3 ubiquitin-protein ligase SMURF2
Sequence	50: MSNPGGRRNG ; PVKLRLTVLC ; AKNLVKKDFF ; RLPDPFAKVV ; VDGSGQCHST ; 100: DTVKNTLDPK ; WNQHYDLYIG ; KSDSVTISVW ; NHKKIHKKQG ; AGFLGCVRLL ; 150: SNAINRLKDT ; GYQRLDLCKL ; GPNDNDTVRG ; QIVVSLQSRD ; RIGTGGQVVD ; 200: CSRLFDNDLP ; DGWEERRTAS ; GRIQYLNHIT ; RTTQWERPTR ; PASEYSSPGR ; 250: PLSCFVDENT ; PITGTNGATC ; GHSSDPRLAE ; RRVRSQRHRN ; YMSRTHLHTP ; 300: PDLPEGYEQR ; TTQQGQVYFL ; HTQTGVSTWH ; DPRVPRDLSN ; INCEELGPLP ; 350: PGWEIRNTAT ; GRVYFVDHNN ; RTTQFTDPRL ; SANLHLVLNR ; QNQLKDQQQQ ; 400: QVVPLCPDDT ; ECLTVPRYKR ; DLVQKLKILR ; QELSQQQPQA ; GHCRIEVSRE ; 450: EIFEESYRQV ; MKMRPKDLWK ; RLMIKFRGEE ; GLDYGGVARE ; WLYLLSHEML ; 500: NPYYGLFQYS ; RDDIYTLQIN ; PDSAVNPEHL ; SYFHFVGRIM ; GMAVFHGHYI ; 550: DGGFTLPFYK ; QLLGKSITLD ; DMELVDPDLH ; NSLVWILEND ; ITGVLDHTFC ; 600: VEHNAYGEII ; QHELKPNGKS ; IPVTEENKKE ; YVRLYVNWRF ; LRGIEAQFLA ; 650: LQKGFNEVIP ; QHLLKTFDEK ; ELELIICGLG ; KIDVSDWKVN ; TRLKHCTPDS ; 700: NVVKWFWKAV ; EFFDEERRAR ; LLQFVTGSSR ; VPLQGFKALQ ; GAAGPRLFTI ; 748: HQIDACTNNL ; PKAHTCFNRI ; DIPPYESYEK ; LYEKLLTAIE ; ETCGFAVE
Sequence Length	748
String	A2A5Z6
Sub Unit	Interacts (via WW domains) with SMAD1. Interacts (via WW domains) with SMAD2 (via PY-motif). Interacts (via WW domains) with SMAD3 (via PY-motif). Interacts with SMAD6. Interacts with SMAD7 (via PY-motif) and TGFBR1; SMAD7 recruits SMURF2 to the TGF-beta receptor and regulates its degradation. Does not interact with SMAD4; SMAD4 lacks a PY-motif. Interacts with AIMP1 (By similarity). Interacts with NDFIP1 and NDFIP2; this interaction activates the E3 ubiquitin-protein ligase.

Uniprot	A2A5Z6
Gene Name	Smurf2
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:66313
Unigene	Mm.340955

Reference	1. "Ubiquitin ligase Smurf1 controls osteoblast activity and bone homeostasis by targeting MEKK2 for degradation." Yamashita M., Ying S.X., Zhang G.M., Li C., Cheng S.Y., Deng C.X., Zhang Y.E. Cell121:101-113(2005) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1) strain: 129/SvJ. 2. "Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.more... PLoS Biol.7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] strain: C57BL/6J. 3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (2005-07) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] 4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1) tissue: Lung. 5. "Tumor necrosis factor promotes Runx2 degradation through up-regulation of Smurf1 and Smurf2 in osteoblasts." Kaneki H., Guo R., Chen D., Yao Z., Schwarz E.M., Zhang Y.E., Boyce B.F., Xing L. J. Biol. Chem.281:4326-4333(2006) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INDUCTION BY TNF SIGNALING PATHWAY ACTIVATION 6. "AIMP1/p43 downregulates TGF-beta signaling via stabilization of smurf2." Lee Y.S., Han J.M., Son S.H., Choi J.W., Jeon E.J., Bae S.-C., Park Y.I., Kim S. Biochem. Biophys. Res. Commun.371:395-400(2008) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STABILIZATION BY SCYE1

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