ELISA Kit for Human Prostaglandin-H2 D-isomerase

Mfr.No.

E0167h

Alternative Names

Beta-trace protein ; Cerebrin-28 ; Glutathione-independent PGD synthase ; Lipocalin-type prostaglandin-D synthase ; Prostaglandin-D2 synthase ; PGD2 synthase ; PDS

Categories

Elisa

Function

Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.

Specificity

Natural and recombinant Human Prostaglandin-H2 D-isomerase

Subcellular Location

Rough endoplasmic reticulum Nucleus membrane Golgi apparatus Cytoplasm perinuclear region Secreted Detected on rough endoplasmic reticulum of arachnoid and menigioma cells. Localized to the nuclear envelope, Golgi apparatus, secretory vesicles and spherical cytoplasmic structures in arachnoid trabecular cells, and to circular cytoplasmic structures in meningeal macrophages and perivascular microglial cells. In oligodendrocytes, localized to the rough endoplasmic reticulum and nuclear envelope. In retinal pigment epithelial cells, localized to distinct cytoplasmic domains including the perinuclear region. Also secreted.

Detection Range

0.78-50ng/mL

Entryname

PTGDS_HUMAN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PTGDS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	92-103%	92-104%	94-102%
EDTA plasma(n=5)	91-106%	93-105%	96-101%	95-100%
heparin plasma(n=5)	94-101%	91-104%	95-102%	93-105%

Method Type

Sandwich

Platform

PF00061

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.3%

Protein Name

Prostaglandin-H2 D-isomerase

Recovery

Recovery was determined by spiking various levels of PTGDS into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	98	92-102
Plasma	99	96-104

Sensitivity

0.34ng/mL

Sequence

50:

MATHHTLWMG ; LALLGVLGDL ; QAAPEAQVSV ; QPNFQQDKFL ; GRWFSAGLAS ;

100:

NSSWLREKKA ; ALSMCKSVVA ; PATDGGLNLT ; STFLRKNQCE ; TRTMLLQPAG ;

150:

SLGSYSYRSP ; HWGSTYSVSV ; VETDYDQYAL ; LYSQGSKGPG ; EDFRMATLYS ;

190:

RTQTPRAELK ; EKFTAFCKAQ ; GFTEDTIVFL ; PQTDKCMTEQ ;

Sequence Length

190

String

P41222

Sub Unit

Monomer.

Uniprot	P41222
Gene Name	PTGDS
Immunogen Species	Homo Sapiens (Human)
Kegg	hsa:5730
Unigene	Hs.446429

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SKU	Supplier No.	Size	Your price	Quantity
BHE13809659	E0167h	96T	$736		Add to Cart

ELISA Kit for Human Prostaglandin-H2 D-isomerase

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