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Home / Assay Kits

E. coli RNA polymerase - for 100 assays

BHE16300224

This product includes 33 ul of 2 uM E.coli RNA Polymerase.

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Tel
+1 866.986.9598
Email
orders@biohippo.com
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Product Manual Datasheet
Protocols Description Assay Protocol
The following assay protocol is based on the 384-well plate assay format (plate type: Matrix 4318 or alike). The reaction volume is 30µl and the final assay volume is 60µl. For 96-well plate assays (plate type: Costar 3915 or alike), the reaction volume is 60µl and the final assay volume is 120µl.
 
1. Reagent preparation:
(1) 10 x DNA template: dilute the 100 x DNA template10-fold with water.
(2) 10 x enzyme: dilute the 100 x RNA polymerase 10-fold with the 1 x Buffer.
(3) 10 x NTP mix: dilute the 100 x NTP mix (50 mM) 10-fold with water.
(4) 1 x fluorescence dye: dilute the 10 x fluorescence dye 10-fold with water.
 
2. Reaction:
The total volume of each reaction mixture is 30µl including 18µl of H2O, 3µl of 10 x Buffer, 3µl of 10 x DNA template, 3µl of 10 x enzyme, 3µl of 10 x NTP mix. Incubate the reaction mixture in a standard black 384-well plate (Matrix 4318) at 37°C for 60 min.
 
3. Detection:
Add 30µl of the 1 x fluorescence dye into the 30µl of the reaction mixture. Incubate for 5 min. Measure the fluorescence intensity at 535 nm using the excitation wavelength at 485 nm.
SKU Supplier No. Size Your price Quantity
BHE16300224 RNAP-100EC 100 assays $421.56
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