JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.
Loding Loading ....
Home / Assay Kits

E. coli DNA ligase - for 100 assays

BHE16300183

This product includes 10 ul of 500 nM E. coli ligase.

Contact us to order
Tel
+1 866.986.9598
Email
orders@biohippo.com
Get a Quote
Request More Infos
Order Now

Credit card payments now incur a 3% fee.

Contact us to order
Tel
+1 866.986.9598
Email
orders@biohippo.com
Get a Quote
Ask Merchant
Request More Infos
Sample Request
Product Manual Datasheet
Protocols Description Assay Protocol using the NAD+ -dependent DNA Ligase Assay Kit
1. Reagent preparation:
10 x DNA: dilute the 100 x DNA with water
100 x enzyme: 100 nM NAD+ -dependent DNA ligase
0.1 mM NAD+ : dilute the 10 mM stock with water
1 x fluorescence dye: dilute the 10 x fluorescence dye 10-fold with water
 
2. Reaction:
The total volume of each reaction mixture is 40 µl including: 27.6 µl of H2O, 4 µl of 10 x buffer (Buffer LS), 4 µl of 10 x DNA, 0.4 µl of 100 x enzyme, and 4 µl of 0.1 mM NAD+ . Incubate the reaction mixture at 37°C for 60 min.
 
3. Detection:
Add 200 µl of Reagent T into the 40 µl of reaction mixture. Then add 20 µl of the 1 x fluorescence dye. Mix the reaction solution and incubate it for 15 min. Measure the fluorescence intensity at 535 nm using the excitation wavelength at 485 nm.
 
 
SKU Supplier No. Size Your price Quantity
BHE16300183 LIG-100EC 100 assays $421.56
Add to Cart